Evidence map›Paper›PMID 39235649›Full record

ArticleBiotechnology letters2024

Homologous expression of Aspergillus niger α-L-rhamnosidase and its application in enzymatic debittering of Ougan juice.

Fei Zhang, Xue Wang, Lixia Pan, Zhao Wang, Jianyong Zheng

Abstract read
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In one paragraph

Article in Biotechnology letters, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Fei ZhangCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Xue WangCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Lixia PanCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Zhao WangCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China.
Jianyong ZhengCollege of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310032, China. zjy821212@zjut.edu.cn.ORCID http://orcid.org/0000-0002-9821-1062

Funding

Application Development Special Key Project of Chongqing cstc2021jscx-jbgsX0002National Natural Science Foundation of China no. 31600639
6 · The paper itself

Abstract

The α-L-rhamnosidase (rha1) gene was homologously expressed in Aspergillus niger strains CCTCC 206047 and CCTCC 206047ΔpyrG, using hygromycin B and auxotrophic as selection markers. The engineered A. niger strains RHA001-1 and RHA003-1 were screened, yielding α-L-rhamnosidase activities of 20.81 ± 0.56 U/mL and 15.35 ± 0.87 U/mL, respectively. The copy numbers of the rha1 gene in strains RHA001-1 and RHA003-1 were found to be 18 and 14, respectively. Correlation analysis between copy number and enzyme activity in the A. niger strains revealed that α-L-rhamnosidase activity increased with the copy number of the rha1 gene. Recombinant α-L-rhamnosidase was utilized for the enzymatic debittering of Ougan juice, and its process conditions were optimized. Furthermore, the primary bitter substance neohesperidin (2.22 g/L) in Ougan juice was converted into hesperetin 7-O-glucoside (1.47 g/L) and hesperidin (0.143 g/L). This study presents a novel approach for the production of food-grade α-L-rhamnosidase and establishes a technical foundation for its application in the beverage industry.

Indexed as

Aspergillus nigerFruit and Vegetable JuicesGlycoside HydrolasesFungal ProteinsGene DosageHesperidinRecombinant Proteinsalpha-L-rhamnosidaseFungal ProteinsGlycoside HydrolasesHesperidinRecombinant ProteinsAspergillus nigerEnzymatic debitteringHomologous expressionOugan juiceα-l-rhamnosidase

Identifiers

PMID39235649

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.