ArticleBiotechnology letters2024
Efficient RNA interference method by feeding in Brachionus plicatilis (Rotifera).
Article in Biotechnology letters, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- A methodology for RNAi gene knockdown in the marine annelid Streblospio benedicti by delivering dsRNA.Development (Cambridge, England) · 2026Article
- A transgenic rotifer-based RNA interference approach for antiviral protection againstFrontiers in microbiology · 2026Article
- Article
- Functional Characterization of TwoInternational journal of molecular sciences · 2025Article
- Dual-mechanism therapy for chronic rhinosinusitis via siRNA targeting of bacterial 16S rRNA and disruption of CFTR-pathogen interactions.Brazilian journal of otorhinolaryngologyArticle
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Authors and funding
11 authors.
Funding
Abstract
Rotifers are small, ubiquitous invertebrate animals found throughout the world and have emerged as a promising model system for studying molecular mechanisms in the fields of experimental ecology, aquatic toxicology, and geroscience. However, the lack of efficient gene expression manipulation techniques has hindered the study of rotifers. In this study, we used the L4440 plasmid with two reverse-oriented T7 promoters, along with RNase-deficient E. coli HT115, to efficiently produce dsRNA and thereby present an efficient feeding-based RNAi method in Brachionus plicatilis. We targeted Bp-Ku70 & Ku80, key proteins in the DNA double-strand breaks repair pathway, and then subjected rotifers to UV radiation. We found that the mRNA expression, fecundity, as well as survival rate diminished significantly as a result of RNAi. Overall, our results demonstrate that the feeding-based RNAi method is a simple and efficient tool for gene knockdown in B. plicatilis, advancing their use as a model organism for biological research.
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Registered trials
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