Evidence map›Paper›PMID 39235646›Full record

ArticleMolecular neurobiology2025

Monosome Stalls the Translation Process Mediated by IGF2BP in Arcuate Nucleus for Puberty Onset Delay.

Yifen Shen, Le Zhang, Tao Yang, Xiaosong Li, Chao Liu, Hongmei Li, Yanping Hu, Hao Shen, Hua Li, Yuriy L Orlov and 2 more

Abstract read
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In one paragraph

Article in Molecular neurobiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yifen Shen *Central Laboratory, Suzhou Bay Clinical College, Xuzhou Medical University, Suzhou Ninth People's Hospital, Soochow University, Suzhou, 215200, Jiangsu, China.
Le Zhang *Jiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in Oncology, Suzhou Vocational Health College, Suzhou, 215009, Jiangsu, China.
Tao Yang *Department of Medical Cosmetology, Suzhou Ninth People's Hospital, Soochow University, Suzhou, 215200, Jiangsu, China.
Xiaosong LiDepartment of Anorectal Surgery, Suzhou Ninth People's Hospital, Suzhou, 215200, Jiangsu, China.
Chao LiuCentral Laboratory, Suzhou Bay Clinical College, Xuzhou Medical University, Suzhou Ninth People's Hospital, Soochow University, Suzhou, 215200, Jiangsu, China.
Hongmei LiShanghai Institute of Precision Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200023, China.
Yanping HuDepartment of Molecular Pathology, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, 450008, Henan, China.
Hao ShenClinical Laboratory, Suzhou Ninth People's Hospital, Suzhou, 215200, Jiangsu, China.
Hua LiJiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in Oncology, Suzhou Vocational Health College, Suzhou, 215009, Jiangsu, China. kaikaixinxin@sjtu.edu.cn.
Yuriy L OrlovThe Digital Health Center, Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Moscow, 119991, Russia. orlov@d-health.institute.
Shasha ZhouDepartment of Endocrinology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, 200040, China. zhoushasha@sjtu.edu.cn.
Yihang ShenCentral Laboratory, Suzhou Bay Clinical College, Xuzhou Medical University, Suzhou Ninth People's Hospital, Soochow University, Suzhou, 215200, Jiangsu, China. devbrother@sjtu.edu.cn.ORCID http://orcid.org/0000-0002-2923-1641

Funding

Gusu Health Talent Training Project GSWS2019087Gusu Health Talent Training Project GSWS2022111Jiangsu Province High-level Innovation and Entrepreneurship Talent Plan JSSCBS20222022National Natural Science Foundation of China 82201797Natural Science Foundation of Shanghai Municipality 22ZR1451900Program of Developing Public Health through Science and Education of Wujiang District, Suzhou wwk202306Russian Science Foundation 24-24-00563Science and Technology Planning Fund of Affiliated Hospital of Xuzhou Medical University XYFM202217Science and Technology Planning Fund of Affiliated Hospital of Xuzhou Medical University XYFY202219Science and Technology Planning Fund of Affiliated Hospital of Xuzhou Medical University XYFY202306Suzhou Science and Technology Planning Project SKJY2021016Suzhou Science and Technology Planning Project SKYD2022023
6 · The paper itself

Abstract

Puberty onset through hypothalamic-pituitary-gonad (HPG) axis as an important reproductive event in postnatal development is initiated from hypothalamic arcuate nucleus (ARC). The growing evidence indicates that translational control also plays an essential role in the final expression of gonadotropin genes. To investigate the role of protein translation and behavior of ribosomes in pubertal onset, the global profiles of transcriptome, single ribosome (monosome), polysome, and tandem mass tag proteome were comprehensively investigated in rat hypothalamic ARCs of different pubertal stages using RNA sequencing, polyribo sequencing, and mass spectrum. Transcriptome-wide enrichments of N6-methyladenosine and IGF2BP2 were investigated using meRIP and RIP sequencing. Monosome was robustly enriched on a large proportion of mRNA in early puberty rats (postnatal day (PND)-25) compared to late puberty (PND-35 and PND-45). Monosome-enriched mRNAs, including HPG axis-related genes, had a large number of upstream ORFs (uORF, < 100 nt) and displayed translational repression in early puberty. Furthermore, insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) could particularly interact with and facilitate monosome to bind with mRNA in early puberty. Finally, ectopic over-expression of IGF2BP2 in hypothalamic ARC via lateral ventricle injection in vivo could recruit monosome to aggregate on mRNA and delay puberty onset. We uncovered a novel regulatory mechanism of IGF2BP2 and monosome for translational control in puberty onset, which shed light on the neuroendocrine regulatory network involved in HPG axis activation.

Indexed as

Arcuate Nucleus of HypothalamusProtein BiosynthesisRibosomesRNA-Binding ProteinsSexual MaturationAnimalsMaleRatsRats, Sprague-DawleyRNA, MessengerTranscriptomeRNA-Binding ProteinsRNA, MessengerARCHypothalamusIGF2BP2MonosomePubertyRibosomeTranslation regulation

Identifiers

PMID39235646

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.