Evidence map›Paper›PMID 39233002›Full record

ArticleJournal of advanced research2025

XYLEM NAC DOMAIN 1 (EjXND1) relieves cold-induced lignification by negatively regulating the EjHB1-EjPRX12 module in loquat fruit.

Zihao Liang, Yanna Shi, Yiqing Huang, Jiao Lu, Mengxue Zhang, Xizhi Cao, Ruoqian Hu, Dongdong Li, Wenbo Chen, Changqing Zhu and 2 more

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In one paragraph

Article in Journal of advanced research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Zihao LiangCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Yanna ShiCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth and Development, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Yiqing HuangCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Jiao LuCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Mengxue ZhangCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Xizhi CaoCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Ruoqian HuCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Dongdong LiCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth and Development, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Wenbo ChenCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth and Development, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Changqing ZhuCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth and Development, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Di WuCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth and Development, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
Kunsong ChenCollege of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth and Development, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. Electronic address: akun@zju.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionLignin is a principal constituent of the secondary cell wall, which plays a role in both plant growth and defensing against stress, such as low temperature and pest infestation. Additionally, it also accumulates in fleshy fruits and negatively affects fruit quality. Red-fleshed loquat is temperature sensitive and exhibits cold-induced lignification. A number of technologies have been developed, for example, Low Temperature Conditioning (LTC) treatment, which has been applied in order to relieve the symptom of cold injury.

objectivesThe present study seeks to elucidate the regulatory mechanism underlying cold-induced lignification in loquat fruit.

methodsThe target genes were isolated through the analysis of transcriptome. The gene function was analyzed by transient transgenic method in tobacco leaves and loquat fruit, respectively, as well as stable overexpression in liverwort. The regulatory mechanism study was achieved by in vitro protein-protein interaction assays, dual-luciferase assay, and EMSA.

resultsIn the present study, the Xylem NAC Domain transcription factor EjXND1 was identified as a repressor of loquat fruit lignification. It was demonstrated that EjXND1 could interact with the characterized lignin activator EjHB1, resulting in a diminution of the activation of EjHB1 on EjPRX12 promoter. Furthermore, two highly methylated regions were identified in the promoter of EjXDN1. One of these regions exhibited a negative correlation between methylation level and EjXND1 expression. Additionally, it was shown that hypermethylation of this region weaken the binding affinity of EjXND1 activators to its promoter.

conclusionThe EjXND1 plays a role in modified Low Temperature Conditioning (mLTC) treatment that alleviates cold-induced lignification in red-fleshed loquat fruit by targeting the EjHB1-EjPRX12 module and EjXND1 is regulated by the dynamic of DNA methylation level in the promoter.

Indexed as

EriobotryaFruitLigninPlant ProteinsTranscription FactorsCold TemperatureGene Expression Regulation, PlantPlant ExtractsPlants, Genetically ModifiedXylemLigninloquat extractPlant ExtractsPlant ProteinsTranscription FactorsDNA methylationFruit qualityLignificationTranscriptional regulationXND1

Identifiers

PMID39233002
PMCPMC12225896

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.