ArticleScientific reports2024
Effect of photobiomodulation therapy with 660 and 980 nm diode lasers on differentiation of periodontal ligament mesenchymal stem cells.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Effect of diode laser irradiation on stemness of dental Tissues - A systematic review.Lasers in medical science · 2025Pooled it
- Light-Based Therapies for Metabolic Modulation of Mesenchymal Stem Cells in Regenerative Dentistry.Lasers in medical science · 2026Review
- In vitro effect of simultaneous irradiation of 635 and 980 nm lasers on proliferation of periodontal ligament mesenchymal cells and their differentiation to osteoblasts.Lasers in medical science · 2026Article
- Review
- Photobiomodulation With 976 nm Diode Laser Enhances Osteoblastic Differentiation of Saos-2 Cells Cultured on Collagen Membranes.Clinical and experimental dental research · 2026Article
- Comparative photobiomodulatory effects of 660 nm, 810 nm, and 940 nm diode lasers on platelet-rich fibrin: an ex vivo study on structural remodeling and VEGF/PDGF-BB release for enhanced periodontal healing.Lasers in medical science · 2025Article
- The impact of photobiomodulation therapy on the survival and differentiation of periodontal ligament mesenchymal stem cells exposed to zoledronic acid.Scientific reports · 2025Article
- Impact of Photobiomodulation on the Pro-Osteogenic Activity of Dental Pulp Mesenchymal Stem/Stromal Cells.International journal of molecular sciences · 2025Article
- Effect of Low-Level Laser Therapy on Periodontal Host Cells and a Seven-Species Periodontitis Model Biofilm.International journal of molecular sciences · 2025Article
- In vitro photobiomodulation enhances human adipose-derived stem cell viability and exosome quality: a novel approach for regenerative medicine.Lasers in medical science · 2025Article
- Low-level laser therapy enhances osteogenic differentiation of gingiva-derived stem cells in 2D and 3D cultures.Scientific reports · 2025Article
- Effect of Photobiomodulation Therapy with Three Wavelengths of Diode Laser on Proliferation and Survival of Stem Cells Derived from Impacted Third Molar Follicles.Journal of lasers in medical sciences · 2025Article
- Effects of photobiomodulation with 450 nm and 635 nm laser wavelengths on the release of TGF-β, BMP-2, FGF-2, and VEGF from dental pulp stem cells.Photochemistry and photobiologyArticle
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aimed to compare the effects of photobiomodulation therapy (PBMT) with 660 and 980 nm diode lasers on differentiation of periodontal ligament mesenchymal stem cells (PDLMSCs). In this in vitro, experimental study, PDLMSCs were obtained from the Iranian Genetic Bank and cultured in osteogenic medium. They were then subjected to irradiation of 660 and 980 nm diode lasers, and their viability was assessed after one, two, and three irradiation cycles using the methyl thiazolyl tetrazolium (MTT) assay. The cells also underwent DAPI staining, cell apoptosis assay by using the Annexin V/PI, Alizarin Red staining, and real-time polymerase chain reaction (PCR) for assessment of the expression of osteogenic genes. Data were analyzed by two-way ANOVA. The two laser groups had no significant difference in cell apoptosis according to the results of DAPI staining. Both laser groups showed higher cell viability in the MTT assay at 4 and 6 days compared with the control group. Annexin V/PI results showed higher cell viability in both laser groups at 4 days compared with the control group. Rate of early and late apoptosis was lower in both laser groups than the control group at 4 days. Necrosis had a lower frequency in 980 nm laser group than the control group on day 6. Alizarin Red staining showed higher cell differentiation in both laser groups after 3 irradiation cycles than the control group. The highest expression of osteopontin (OPN), osteocalcin (OCN), and Runt-related transcription factor 2 (RUNX2) was noted in 660 nm laser group with 3 irradiation cycles at 14 days, compared with the control group. PBMT with 660 and 980 nm diode lasers decreased apoptosis and significantly increased PDLMSC differentiation after 3 irradiation cycles.
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