ArticleMolecular biology reports2024
Leukocyte-and Platelet-Rich Fibrin for enhanced tissue repair: an in vitro study characterizing cellular composition, growth factor kinetics and transcriptomic insights.
Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed.
- Comparative Outcome Analysis of Leukocyte-Rich Platelet-rich Plasma, Leukocyte-Poor Platelet-rich Plasma, Injectable Platelet-rich Fibrin and Corticosteroid Injections in Periarthritis of the Shoulder: A Double-Blinded, Four-Arm Randomized Controlled Trial.Journal of orthopaedic case reports · 2026Article
- Review
- Fibrin-Based Biomaterials in Wound Healing and Soft Tissue Regeneration: Biological Mechanisms and Clinical Applications.Gels (Basel, Switzerland) · 2026Review
- Platelet-rich fibrin-derived extracellular vesicles: emerging biological mediators in periodontal regeneration.Odontology · 2026Review
- The Role of Biologically Active Materials in Peri-Implant Diseases.Journal of clinical medicine · 2026Review
- Therapeutic Effect of Photobiomodulation and Single Intratendinous Injection at the Lesion Site of Platelet-Rich Fibrin in Inducted Tendinopathy.Journal of biophotonics · 2026Article
- Article
- The influence of platelet-rich fibrin combined with hydroxychloroquine on the inflammatory response; bone metabolism; and cell differentiation ability in temporomandibular joint osteoarthritis.Journal of medical biochemistry · 2026Article
- Biomolecule-functionalized dental implant surfaces: Towards augmenting soft tissue integration.Bioactive materials · 2025Review
- Effect of Leukocyte- and Platelet-Rich Fibrin on Peri-Implant Mucosal Thickness in Edentulous Patients Treated with Mandibular Implant-Retained Overdentures: A Randomized Controlled Trial.Journal of clinical medicine · 2025Article
- Extended PRF: Impact of Heat on Gene Expression in Gingival Fibroblasts.International journal of molecular sciences · 2025Article
- Advancing bone regeneration: Clinical implications of synthetic biomaterials and fibrin derivatives.World journal of orthopedics · 2025Article
- Beyond the clot: how the biomolecular landscape of platelet-rich fibrin directs fibroblast functions.Frontiers in oral health · 2025Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundLeukocyte- and platelet-rich fibrin (L-PRF) is an autologous platelet concentrate, prepared by centrifugation of blood and consisting of a dense fibrin network with incorporated leukocytes and platelets. This study aims to perform an in-depth analysis of the cells, growth factors, and transcriptome of L-PRF. METHODS AND
resultsFresh, 1 week and 2 weeks cultured human L-PRF membranes and liquid L-PRF glue were characterized on cellular and transcriptional level using flow cytometry (n = 4), single-cell RNA sequencing (n = 5) and RT-qPCR. Growth factor kinetics were investigated using ELISA (EGF, VEGF, PDGF-AB, TGF-β1, bFGF). L-PRF contained a large number of viable cells (fresh 97.14 ± 1.09%, 1 week cultured 93.57 ± 1.68%), mainly granulocytes in fresh samples (53.9 ± 19.86%) and T cells in cultured samples (84.7 ± 6.1%), confirmed with scRNA-seq. Monocytes differentiate to macrophages during 1 week incubation. Specifically arterial L-PRF membranes were found to release significant amounts of VEGF, EGF, PDGF-AB and TGF-β1.
conclusionWe characterized L-PRF using in vitro experiments, to obtain an insight in the composition of the material including a possible mechanistic role for tissue healing. This was the first study characterizing L-PRF at a combined cellular, proteomic, and transcriptional level.
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Registered trials
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