Evidence map›Paper›PMID 39230280›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2024

Toward Analysis at the Point of Need: A Digital Microfluidic Approach to Processing Multi-Source Sexual Assault Samples.

Mohamed Elsayed, Leticia Bodo, Christine Gaoiran, Palig Keuhnelian, Advikaa Dosajh, Vivienne Luk, Melissa Schwandt, Julie L French, Alpana Ghosh, Barbara Erickson and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Mohamed ElsayedInstitute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, M5S 3E2, Canada.
Leticia BodoDonnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, ON, M5S 3E1, Canada.
Christine GaoiranForensic Science Department, University of Toronto Mississauga, 4th floor, Terrence Donnelly Health Sciences Complex, 3359 Mississauga Rd., Mississauga, ON, L5L 1C6, Canada.
Palig KeuhnelianForensic Science Department, University of Toronto Mississauga, 4th floor, Terrence Donnelly Health Sciences Complex, 3359 Mississauga Rd., Mississauga, ON, L5L 1C6, Canada.
Advikaa DosajhForensic Science Department, University of Toronto Mississauga, 4th floor, Terrence Donnelly Health Sciences Complex, 3359 Mississauga Rd., Mississauga, ON, L5L 1C6, Canada.
Vivienne LukForensic Science Department, University of Toronto Mississauga, 4th floor, Terrence Donnelly Health Sciences Complex, 3359 Mississauga Rd., Mississauga, ON, L5L 1C6, Canada.
Melissa SchwandtANDE Corporation, 1860 Industrial Circle, Suite A, Longmont, CO, 80501, USA.
Julie L FrenchANDE Corporation, 1860 Industrial Circle, Suite A, Longmont, CO, 80501, USA.
Alpana GhoshCentre of Forensic Sciences, 25 Morton Shulman Avenue, Toronto, ON, M3M 0B1, Canada.
Barbara EricksonCentre of Forensic Sciences, 25 Morton Shulman Avenue, Toronto, ON, M3M 0B1, Canada.
Amanda G CharlesworthCentre of Forensic Sciences, 25 Morton Shulman Avenue, Toronto, ON, M3M 0B1, Canada.
Jonathan MillmanCentre of Forensic Sciences, 25 Morton Shulman Avenue, Toronto, ON, M3M 0B1, Canada.
Aaron R WheelerInstitute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, M5S 3E2, Canada.ORCID 0000-0001-5230-7475

Funding

Canadian Natural Science and Engineering Research Council ALLRP 562031 - 21
6 · The paper itself

Abstract

Forensic case samples collected in sexual assaults typically contain DNA from multiple sources, which complicates short-tandem repeat (STR) profiling. These samples are typically sent to a laboratory to separate the DNA from sperm and non-sperm sources prior to analysis. Here, the automation and miniaturization of these steps using digital microfluidics (DMF) is reported, which may eventually enable processing sexual assault samples outside of the laboratory, at the point of need. When applied to vaginal swab samples collected up to 12 h post-coitus (PC), the new method identifies single-source (male) STR profiles. When applied to samples collected 24-72 h PC, the method identifies mixed STR profiles, suggesting room for improvement and/or potential for data deconvolution. In sum, an automated, miniaturized sample pre-processing method for separating the DNA contained in sexual assault samples is demonstrated. This type of automated processing using DMF, especially when combined with Rapid DNA Analysis, has the potential to be used for processing of sexual assault samples in hospitals, police offices, and other locations outside of the laboratory.

Indexed as

MicrofluidicsSex OffensesSpecimen HandlingDNADNA FingerprintingFemaleHumansMaleMicrosatellite RepeatsDNAdifferential digestiondigital microfluidicsrapid DNA analysis

Identifiers

PMID39230280
PMCPMC11538644

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.