Evidence map›Paper›PMID 39229060›Full record

ArticlebioRxiv : the preprint server for biology2024

Dynamins combine mechano-constriction and membrane remodeling to enable two-step mitochondrial fission via a 'snap-through' instability.

Haleh Alimohamadi, Elizabeth Wei-Chia Luo, Rena Yang, Shivam Gupta, Kelsey A Nolden, Taraknath Mandal, R Blake Hill, Gerard C L Wong

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Haleh AlimohamadiDepartment of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90025, USA.ORCID 0000-0001-6576-2426
Elizabeth Wei-Chia LuoDepartment of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90025, USA.
Rena YangDepartment of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90025, USA.
Shivam GuptaDepartment of Physics, Indian Institute of Technology Kanpur, Kanpur, 208016, India.
Kelsey A NoldenDepartment of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, 53226, USA.
Taraknath MandalDepartment of Physics, Indian Institute of Technology Kanpur, Kanpur, 208016, India.
R Blake HillDepartment of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, 53226, USA.
Gerard C L WongDepartment of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90025, USA.

Funding

A Synchrotron Radiation Structural Biology ResourcesP30GM133894 · NIGMS · STANFORD UNIVERSITY · PI Aina E. Cohen, KEITH O HODGSON · 2020 to 2026
$43.3M
Vascular Biology Training GrantT32HL069766 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LUSIS, ALDONS JAKE · 2002 to 2022
$7.4M
Structural Biology of Mitochondrial FissionR01GM067180 · NIGMS · MEDICAL COLLEGE OF WISCONSIN · PI HILL, R BLAKE · 2004 to 2022
$6.0M
Medical Scientist Training ProgramT32GM080202 · NIGMS · MEDICAL COLLEGE OF WISCONSIN · PI BARBIERI, JOSEPH T, SALZMAN, NITA H · 2010 to 2024
$5.7M
Systems Genetics Dissection of Non-alcoholic SteatohepatitisR01DK117850 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Aldons Jake Lusis · 2019 to 2026
$4.6M
American Heart Association-American Stroke Association 966662NHLBI NIH HHS T32 HL069766NIDDK NIH HHS R01 DK117850NIGMS NIH HHS P30 GM133894NIGMS NIH HHS R01 GM067180NIGMS NIH HHS T32 GM080202
6 · The paper itself

Abstract

Mitochondrial fission is controlled by dynamin proteins, the dysregulation of which is correlated with diverse diseases. Fission dynamins are GTP hydrolysis-driven mechanoenzymes that self-oligomerize into helical structures that constrict membrane to achieve fission, but details are not well understood. However, dynamins can also remodel membranes by inducing negative Gaussian curvature, the type of curvature required for completion of fission. Here, we examine how these drastically different mechanisms synergistically exert their influences on a membrane, via a mechanical model calibrated with small-angle X-ray scattering structural data. We find that free dynamin can trigger a "snap-through instability" that enforces a shape transition from an oligomer-confined cylindrical membrane to a drastically narrower catenoid-shaped neck within the spontaneous hemi-fission regime, in a manner that depends critically on the length of the confined tube. These results indicate how the combination of dynamin assembly, and paradoxically disassembly, can lead to diverse pathways to scission.

Identifiers

PMID39229060
PMCPMC11370335

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.