ArticleThe EMBO journal2024
Elucidating the assembly of gas vesicles by systematic protein-protein interaction analysis.
Article in The EMBO journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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Who cites it
5 citing papers in PubMed.
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Emerging Nanoplatforms are Effective Against Tumor Hypoxia.International journal of nanomedicine · 2026Review
- Temporal gene regulation enables controlled expression of gas vesicles and preserves bacterial viability.Nature communications · 2025Article
- Directed Evolution of Acoustic Reporter Genes Using High-Throughput Acoustic Screening.ACS synthetic biology · 2024Article
- Structural biology of microbial gas vesicles: historical milestones and current knowledge.Biochemical Society transactions · 2024Review
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Authors and funding
6 authors.
Funding
Abstract
Gas vesicles (GVs) are gas-filled microbial organelles formed by unique 3-nm thick, amphipathic, force-bearing protein shells, which can withstand multiple atmospheric pressures and maintain a physically stable air bubble with megapascal surface tension. However, the molecular process of GV assembly remains elusive. To begin understanding this process, we have devised a high-throughput in vivo assay to determine the interactions of all 11 proteins in the pNL29 GV operon. Complete or partial deletions of the operon establish interdependent relationships among GV proteins during assembly. We also examine the tolerance of the GV assembly process to protein mutations and the cellular burdens caused by GV proteins. Clusters of GV protein interactions are revealed, proposing plausible protein complexes that are important for GV assembly. We anticipate our findings will set the stage for designing GVs that efficiently assemble in heterologous hosts during biomedical applications.
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Registered trials
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