Evidence map›Paper›PMID 39226360›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2024

Superresolution imaging of antibiotic-induced structural disruption of bacteria enabled by photochromic glycomicelles.

Xi-Le Hu, Hui-Qi Gan, Wen-Zhen Gui, Kai-Cheng Yan, Jonathan L Sessler, Dong Yi, He Tian, Xiao-Peng He

Abstract read
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In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xi-Le Hu *Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
Hui-Qi Gan *Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
Wen-Zhen Gui *Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
Kai-Cheng YanKey Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
Jonathan L SesslerDepartment of Chemistry, The University of Texas at Austin, Austin, TX 78712-1224.ORCID 0000-0002-9576-1325
Dong YiResearch Center for Systems Biosynthesis, China State Institute of Pharmaceutical Industry, National Key Laboratory of Lead Druggability Research, Shanghai 201203, China.
He TianKey Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
Xiao-Peng HeKey Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.

Funding

CAS | State Key Laboratory of Drug Research, Chinese Academy of Sciences (SKLAD) SKLDR-2023-KF-10Ministry of Education Key Laboratory on Signaling Regulation 2023-MEKLLC-MS/ZD-00*MOE | Fundamental Research Funds for the Central Universities (Fundamental Research Fund for the Central Universities) 222201717003MOST | National Natural Science Foundation of China (NSFC) 92253306 and 82130099Programme of Introducing Talents of Disciplines to Universities B16017Shanghai Municipal Science and Technology 2018SHZDZX03Welch Foundation (The Welch Foundation) F-0018
6 · The paper itself

Abstract

Bacterial evolution, particularly in hospital settings, is leading to an increase in multidrug resistance. Understanding the basis for this resistance is critical as it can drive discovery of new antibiotics while allowing the clinical use of known antibiotics to be optimized. Here, we report a photoactive chemical probe for superresolution microscopy that allows for the in situ probing of antibiotic-induced structural disruption of bacteria. Conjugation between a spiropyran (SP) and galactose via click chemistry produces an amphiphilic photochromic glycoprobe, which self-assembles into glycomicelles in water. The hydrophobic inner core of the glycomicelles allows encapsulation of antibiotics. Photoirradiation then serves to convert the SP to the corresponding merocyanine (MR) form. This results in micellar disassembly allowing for release of the antibiotic in an on-demand fashion. The glycomicelles of this study adhere selectively to the surface of a Gram-negative bacterium through multivalent sugar-lectin interaction. Antibiotic release from the glycomicelles then induces membrane collapse. This dynamic process can be imaged in situ by superresolution spectroscopy owing to the "fluorescence blinking" of the SP/MR photochromic pair. This research provides a high-precision imaging tool that may be used to visualize how antibiotics disrupt the structural integrity of bacteria in real time.

Indexed as

Anti-Bacterial AgentsBenzopyransIndolesMicellesNitro CompoundsPyrimidinonesAnti-Bacterial AgentsBenzopyransIndolesmerocyanineMicellesNitro CompoundsPyrimidinonesspiropyranantibioticsfluorescence imagingphotochromismsuperbugssuperresolution

Identifiers

PMID39226360
PMCPMC11406247

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.