Evidence map›Paper›PMID 39226319›Full record

ArticlePloS one2024

Mmu-let-7a-5p inhibits macrophage apoptosis by targeting CASP3 to increase bacterial load and facilities mycobacterium survival.

Xuehua Zhan, Wenqi Yuan, Rong Ma, Yueyong Zhou, Guangxian Xu, Zhaohui Ge

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xuehua ZhanDepartment of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China.ORCID 0000-0002-3189-8648
Wenqi YuanDepartment of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China.
Rong MaDepartment of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China.
Yueyong ZhouClinical Medicine School, Ningxia Medical University, Yinchuan, Ningxia, China.
Guangxian XuThe First Dongguan Affiliated Hospital, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, School of Medical Technology, Guangdong Medical University, Dongguan, China.
Zhaohui GeDepartment of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China.ORCID 0000-0001-5167-4601

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have been trying to find a miRNA that can specifically regulate the function of mycobacterial host cells to achieve the purpose of eliminating Mycobacterium tuberculosis. The purpose of this study is to investigate the regulation of mmu-let-7a-5p on macrophages apoptosis and its effect on intracellular BCG clearance. After a series of in vitro experiments, we found that mmu-let-7a-5p could negatively regulate the apoptosis of macrophages by targeting Caspase-3. The extrinsic apoptosis signal axis TNFR1/FADD/Caspase-8/Caspase-3 was inhibited after BCG infection. Up-regulated the expression level of mmu-let-7a-5p increase the cell proliferation viability and inhibit apoptosis rate of macrophages, but down-regulated its level could apparently reduce the bacterial load of intracellular Mycobacteria and accelerate the clearance of residual Mycobacteria effectively. Mmu-let-7a-5p has great potential to be utilized as an optimal candidate exosomal loaded miRNA for anti-tuberculosis immunotherapy in our subsequent research.

Indexed as

ApoptosisBacterial LoadCaspase 3MacrophagesMicroRNAsAnimalsCell ProliferationMiceMycobacterium bovisMycobacterium tuberculosisRAW 264.7 CellsCasp3 protein, mouseCaspase 3MicroRNAs

Identifiers

PMID39226319
PMCPMC11371246

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.