Protocol for volume correlative light X-ray and electron microscopy of endothelial cells in mouse tissue.

Natalia Reglero-Real, Lorena Pérez-Gutiérrez, Rebeca S Saleeb, Sussan Nourshargh, Lucy Collinson, Azumi Yoshimura

Abstract read

In one paragraph

Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Natalia Reglero-RealCentre for Microvascular Research, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, EC1M 6BQ London, UK; Departamento e Instituto de Biología Molecular (IUBM, UAM), Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, UAM-CSIC, Campus de Cantoblanco, 28049 Madrid, Spain. Electronic address: nreglero@cbm.csic.es.

Lorena Pérez-GutiérrezCentre for Microvascular Research, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, EC1M 6BQ London, UK; Cancer Immunology and Immunotherapy Lab, Center for Cooperative Research in Biosciences (CIC BioGUNE), Basque Research and Technology Alliance (BRTA), Derio, 48160 Bizkaia, Spain.

Rebeca S SaleebCentre for Microvascular Research, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, EC1M 6BQ London, UK; Institute of Regeneration and Repair, University of Edinburgh, EH16 4UU Edinburgh, UK.

Sussan NoursharghCentre for Microvascular Research, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, EC1M 6BQ London, UK.

Lucy CollinsonElectron Microscopy Science Technology Platform, The Francis Crick Institute, NW1 1AT London, UK. Electronic address: lucy.collinson@crick.ac.uk.

Azumi YoshimuraElectron Microscopy Science Technology Platform, The Francis Crick Institute, NW1 1AT London, UK; Graduate School of Medicine, Yamaguchi University, Ube 755-8505, Japan. Electronic address: azumi-y@yamaguchi-u.ac.jp.

Funding

Wellcome Trust

6 · The paper itself

Abstract

Correlative light and electron microscopy (CLEM) greatly facilitate capturing the ultrastructure of spatially and/or temporally rare events. Here, we present a protocol for targeting regions of interests (ROIs) in tissue endothelial cells (ECs) using X-ray micro-computed tomography (μCT). We describe steps for ROI targeting guided by vasculature patterns and positions of EC nuclei visualized by light and X-ray microscopy. The protocol is applicable to thin or translucent tissues that contain defined landmarks visible in both light and X-ray microscopy. For complete details on the use and execution of this protocol, please refer to Reglero-Real et al.

Indexed as

Endothelial CellsMicroscopy, ElectronAnimalsMiceMicroscopyX-Ray MicrotomographyCell BiologyImmunologyMicroscopy

Identifiers

PMID39226173

DOI10.1016/j.xpro.2024.103257

PMCPMC11419916

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.