Evidence map›Paper›PMID 39224820›Full record

ArticlePeerJ2024

Development and evaluation of a rapid visual loop-mediated isothermal amplification assay for the

Minyi Lin, Pu Wang, Bingyun Lu, Ming Jin, Jiasheng Tan, Wei Liu, Jing Yuan, Xiaomou Peng, Ye Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Minyi Lin *Department of Infectious Diseases, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, China.
Pu Wang *Department of Gastroenterology, Guangdong Provincial Key Laboratory of Gastroenterology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Bingyun LuIntegrative Microecology Center, Shenzhen Key Laboratory of Gastrointestinal Microbiota and Disease, Shenzhen Clinical Research Center for Digestive Disease, Shenzhen Technology Research Center of Gut Microbiota Transplantation, Shenzhen Hospital, Southern Medical University, Shenzhen, China.
Ming JinIntegrative Microecology Center, Shenzhen Key Laboratory of Gastrointestinal Microbiota and Disease, Shenzhen Clinical Research Center for Digestive Disease, Shenzhen Technology Research Center of Gut Microbiota Transplantation, Shenzhen Hospital, Southern Medical University, Shenzhen, China.
Jiasheng TanDepartment of Gastroenterology, SongShan Lake Central Hospital of Dongguan City, Dongguan, Guangdong, China.
Wei LiuInstitute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Jing YuanInstitute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China.
Xiaomou PengDepartment of Infectious Diseases, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, China.
Ye ChenIntegrative Microecology Center, Shenzhen Key Laboratory of Gastrointestinal Microbiota and Disease, Shenzhen Clinical Research Center for Digestive Disease, Shenzhen Technology Research Center of Gut Microbiota Transplantation, Shenzhen Hospital, Southern Medical University, Shenzhen, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: The Methods: Three sets of primers were designed and optimized to amplify the Results: At a temperature of 66 °C, the target DNA was successfully amplified with a set of primers designated, and visualized within 60 min. Under the same conditions, the target DNA was not amplified with the Conclusion: LAMP method is an effective technique for the rapid and visual detection of the

Indexed as

Bacterial ToxinsClostridioides difficileClostridium InfectionsEnterotoxinsFecesNucleic Acid Amplification TechniquesSensitivity and SpecificityAdultDNA PrimersHumansMiddle AgedMolecular Diagnostic TechniquesPolymerase Chain ReactionBacterial ToxinsDNA PrimersEnterotoxinstcdA protein, Clostridium difficileClostridioides difficileLoop-mediated isothermal amplificationtcdA geneVisual detection

Identifiers

PMID39224820
PMCPMC11368091

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.