Evidence map›Paper›PMID 39224784›Full record

ArticleNanophotonics (Berlin, Germany)2024

Highly sensitive volumetric single-molecule imaging.

Le-Mei Wang, Jiah Kim, Kyu Young Han

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nanophotonics (Berlin, Germany), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Le-Mei WangCREOL, The College of Optics and Photonics, University of Central Florida, Orlando, FL, USA.
Jiah KimDepartment of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Kyu Young HanCREOL, The College of Optics and Photonics, University of Central Florida, Orlando, FL, USA.ORCID https://orcid.org/0000-0002-1448-3125

Funding

Super-multiplexed fluorescence nanoscopy for imaging-based proteomicsR35GM138039 · NIGMS · UNIVERSITY OF CENTRAL FLORIDA · PI Kyu Young Han · 2020 to 2026
$2.2M
NIGMS NIH HHS R35 GM138039
6 · The paper itself

Abstract

Volumetric subcellular imaging has long been essential for studying structures and dynamics in cells and tissues. However, due to limited imaging speed and depth of field, it has been challenging to perform live-cell imaging and single-particle tracking. Here we report a 2.5D fluorescence microscopy combined with highly inclined illumination beams, which significantly reduce not only the image acquisition time but also the out-of-focus background by ∼2-fold compared to epi-illumination. Instead of sequential

Indexed as

extended depth-of-fieldPSF engineeringsingle-moleculessingle-particle trackingsuper-resolution imagingvolumetric imaging

Identifiers

PMID39224784
PMCPMC11366074

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.