ArticleGalen medical journal2024
In Vitro Effects of Photobiomodulation with 660 Nm Laser and Vitamin D on Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells.
Article in Galen medical journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Innovative photobiomodulation-enhanced T-PRF in posterior mandibular fractures: a randomized pilot study.BMC oral health · 2026Trial
- Effect of Photobiomodulation with 980nm Diode Laser and Vitamin D on Proliferation and Osteoblastic Differentiation of Periodontal Ligament Stem Cells : Effects of Photobiomodulation and Vitamin D on Differentiation of StemCells.Galen medical journal · 2024Article
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5 authors.
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Abstract
backgroundMesenchymal stem cells (MSCs) can be found inside the human periodontal ligament. Application of vitamin D and photobiomodulation for regulation of the proliferation of MSCs and bone differentiation have been recently considered in cell engineering. This study is performed to evaluate the effects of photobiomodulation with 660 nm laser exposure and vitamin D on human periodontal ligament stem cells (HPDLSCs) and their osteoblastic differentiation properties. MATERIALS AND
methodsThis study, was an in vitro experimental study performed on HPDLSCs in six groups of (I) control cells in the culture medium with no intervention, (II) addition of 10-7 mol vitamin D to the medium, (III) 660 nm diode laser exposure in 3 J/cm2 density of energy, (IV) 660 nm diode laser exposure in 3 J/cm2 density of energy + addition of 10-7 mol vitamin D to the medium, (V) 660 nm diode laser exposure in 5 J/cm2 density of energy, and (VI) 660 nm diode laser exposure in 5 J/cm2 density of energy + addition of 10-7 mol vitamin D to the medium. after 24 hours of the last exposure, cell viability had been assessed by methyl thiazolyl tetrazolium assay. The expression of Runt-related transcription factor 2 (RUNX2), osteopontin (OPN), alkaline phosphatase (ALP), and osteocalcin (OCN) genes was also assessed by reverse transcription-polymerase chain reaction, then Alizarin red staining was used to assess calcification.
resultsCombined use of 660 nm laser with 3 and 5 J/cm2 density of energy and 10-7 mol vitamin D significantly increased cell viability, osteoblastic differentiation by upregulation of RUNX2, ALP, OPN, and OCN, and calcification (P0.05).
conclusionThe results showed that combined use of vitamin D3 and irradiation of 660 nm laser with 3 J/cm2 and particularly 5 J/cm2 energy density increased the viability of HPDLSCs and enhanced their osteoblastic differentiation.
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