Evidence map›Paper›PMID 39224379›Full record

ArticleHeliyon2024

Alternation of gene expression in brain-derived exosomes after cerebral ischemic preconditioning in mice.

He Li, Xiaoxi Zhang, Hongye Xu, Hanchen Liu, Yongxin Zhang, Lei Zhang, Yu Zhou, Yongwei Zhang, Jianmin Liu, Mei Jing and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

He LiEmergency Department, Naval Medical Center of PLA, Naval Medical University, Shanghai, China.
Xiaoxi ZhangNeurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China.
Hongye XuNeurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China.
Hanchen LiuNeurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China.
Yongxin ZhangNeurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China.
Lei ZhangNeurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China.
Yu ZhouNeurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China.
Yongwei ZhangNeurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China.
Jianmin LiuNeurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China.
Mei JingEmergency Department, Naval Medical Center of PLA, Naval Medical University, Shanghai, China.
Ping ZhangNeurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China.
Pengfei YangNeurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aims: Cerebral ischemic preconditioning is a neuroprotective therapy against cerebral ischemia and ischemia-reperfusion injury. This study aims to demonstrate the alternation of gene expression in exosomes from brain tissue of mice after ischemic preconditioning and their potential functions. Methods: Ten mice were divided into the sham and the cerebral ischemic preconditioning groups. Their brain tissues were harvested, from which the exosomes were extracted. The characteristics and protective effects of exosomes were evaluated. Whole transcriptome sequencing was used to demonstrate the gene expression discrepancy between the exosomes from the two groups of mice brains. Volcano graphs and heatmaps were used to picture the difference in expression quantity of mRNA, lncRNA, and circRNA. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to demonstrate the functions of differentially expressed RNAs. Results: Exosomes were successfully extracted, and those from the cerebral ischemic preconditioning group had better protective effects on cells that received oxygen-glucose deprivation and restoration injury. A total of 306 mRNAs and 374 lncRNAs were significantly upregulated, and 320 mRNAs and 405 lncRNAs were significantly downregulated in the preconditioning group. No circRNAs were differentially expressed between the two groups. GO and KEGG pathway analysis indicated that the functions of differentially expressed RNAs were related to both neural protective and injurious effects. Conclusion: The brain-derived exosomes may participate in the neuroprotective effect of cerebral ischemic preconditioning. Thorough research is necessary to investigate exosome functions derived from the ischemic preconditioned brain.

Indexed as

Cerebral ischemia-reperfusion injuryCerebral ischemic preconditioningExosomesNeuroprotectionWhole transcriptome sequencing

Identifiers

PMID39224379
PMCPMC11367060

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.