Evidence map›Paper›PMID 39223570›Full record

ArticleBMC research notes2024

Validation of a direct multiplex real-time reverse transcription PCR assay for rapid detection of African swine fever virus using swine field samples in Vietnam.

Hiroaki Shirafuji, Tatsuya Nishi, Takehiro Kokuho, Hoang Vu Dang, Anh Duc Truong, Tomoya Kitamura, Mizuki Watanabe, Ha Thi Thanh Tran, Kentaro Masujin

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hiroaki Shirafuji *Division of Transboundary Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo, 187-0022, Japan.
Tatsuya Nishi *Division of Transboundary Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo, 187-0022, Japan.
Takehiro KokuhoDivision of Transboundary Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo, 187-0022, Japan.
Hoang Vu DangDepartment of Biochemistry and Immunology, National Institute of Veterinary Research, 86, Truong Chinh, Dong Da, Hanoi, Vietnam.
Anh Duc TruongDepartment of Biochemistry and Immunology, National Institute of Veterinary Research, 86, Truong Chinh, Dong Da, Hanoi, Vietnam.
Tomoya KitamuraDivision of Transboundary Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo, 187-0022, Japan.
Mizuki WatanabeDivision of Transboundary Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo, 187-0022, Japan.
Ha Thi Thanh TranDepartment of Biochemistry and Immunology, National Institute of Veterinary Research, 86, Truong Chinh, Dong Da, Hanoi, Vietnam. thanhhavty@yahoo.com.
Kentaro MasujinDivision of Transboundary Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo, 187-0022, Japan. masujin@affrc.go.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThis study validates a direct multiplex real-time reverse transcription polymerase chain reaction (rRT-PCR) assay which was previously established for enabling rapid and simultaneous detection of African swine fever (ASF) virus (ASFV) and classical swine fever virus. The assay eliminates the need for viral nucleic acid purification using a buffer system for crude extraction and an impurity-tolerant enzyme. However, the assay had not yet been validated using field samples of ASFV-infected pigs. Therefore, to address this gap, we tested 101 samples collected from pigs in Vietnam during 2018 and 2021 for validation.

resultsThe rRT-PCR assay demonstrated a diagnostic sensitivity of 98.8% and a specificity of 100%. Remarkably, crude samples yielded results comparable to those of purified samples, indicating the feasibility of using crude samples without compromising accuracy in ASFV detection. Our findings emphasize the effectiveness of the rRT-PCR assay for the prompt and accurate diagnosis of both swine fever viruses, which is essential for effective disease prevention and control in swine populations.

Indexed as

African Swine FeverAfrican Swine Fever VirusReal-Time Polymerase Chain ReactionSensitivity and SpecificityAnimalsMultiplex Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionSwineVietnamAfrican swine feverCrude samplesMultiplex real-time RT-PCRValidation

Identifiers

PMID39223570
PMCPMC11370023

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.