Evidence map›Paper›PMID 39223176›Full record

ArticleScientific reports2024

Drug resistance and genomic variations among Mycobacterium tuberculosis isolates from The Nile Delta, Egypt.

May S Soliman, Chungyi H Hansen, Mostafa Hanafy, Sherine Shawky, Heba Rashed, Mohamed Abdullah, Noha Salah Soliman, Maha A Gad, Sahar Khairat, Amani El-Kholy and 1 more

Abstract read
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In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

May S SolimanDepartment of Clinical and Chemical Pathology, Kasr Al Aini, Faculty of Medicine, Cairo University, Giza, Egypt. may.mohmed@kasralainy.edu.eg.
Chungyi H HansenDepartment of Pathobiological Sciences, University of Wisconsin-Madison, Madison, USA.
Mostafa HanafyDepartment of Pathobiological Sciences, University of Wisconsin-Madison, Madison, USA.
Sherine ShawkyMicrobiology Department, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Heba RashedClinical Pathology Department, Faculty of Medicine, Assiut University, Assiut, Egypt.
Mohamed AbdullahCentral Public Health Laboratories, Ministry of Health and Population, Cairo, Egypt.
Noha Salah SolimanDepartment of Clinical and Chemical Pathology, Kasr Al Aini, Faculty of Medicine, Cairo University, Giza, Egypt.
Maha A GadDepartment of Clinical and Chemical Pathology, Kasr Al Aini, Faculty of Medicine, Cairo University, Giza, Egypt.
Sahar KhairatDepartment of Clinical and Chemical Pathology, Kasr Al Aini, Faculty of Medicine, Cairo University, Giza, Egypt.
Amani El-KholyDepartment of Clinical and Chemical Pathology, Kasr Al Aini, Faculty of Medicine, Cairo University, Giza, Egypt.
Adel M TalaatDepartment of Pathobiological Sciences, University of Wisconsin-Madison, Madison, USA.

Funding

Tuberculosis Immunopathogenesis During Superinfection with SARS-CoV2R01AI173411 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI ADEL M TALAAT · 2023 to 2026
$3.0M
NIAID NIH HHS R01 AI173411US-Egypt STDF program, Cycle 19, USAID-STDF, Egypt Project ID:42693/No. 20000010565
6 · The paper itself

Abstract

Tuberculosis is a global public health concern. Earlier reports suggested the emergence of high rates of drug resistant tuberculosis in Egypt. This study included 102 isolates of Mycobacterium tuberculosis collected from two reference laboratories in Cairo and Alexandria. All clinical isolates were sub-cultured on Löwenstein-Jensen medium and analyzed using both BD BACTEC MGIT 960 SIRE Kit and standard diffusion disk assays to identify the antibiotic sensitivity profile. Extracted genomic DNA was subjected to whole genome sequencing (WGS) using Illumina platform. Isolates that belong to lineage 4 represented > 80%, while lineage 3 represented only 11% of the isolates. The percentage of drug resistance for the streptomycin, isoniazid, rifampicin and ethambutol were 31.0, 17.2, 19.5 and 20.7, respectively. Nearly 47.1% of the isolates were sensitive to the four anti-tuberculous drugs, while only one isolate was resistant to all four drugs. In addition, several new and known mutations were identified by WGS. High rates of drug resistance and new mutations were identified in our isolates. Tuberculosis control measures should focus on the spread of mono (S, I, R, E)- and double (S, E)-drug resistant strains present at higher rates throughout the whole Nile Delta, Egypt.

Indexed as

Antitubercular AgentsMicrobial Sensitivity TestsMycobacterium tuberculosisTuberculosis, Multidrug-ResistantWhole Genome SequencingAdultDrug Resistance, BacterialDrug Resistance, Multiple, BacterialEgyptFemaleGenetic VariationGenome, BacterialHumansIsoniazidMaleMiddle AgedAntitubercular AgentsIsoniazidStreptomycinDrug resistanceEgyptGenome-wide variationsLineagesTuberculosis

Identifiers

PMID39223176
PMCPMC11369133

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.