Evidence map›Paper›PMID 39221662›Full record

ArticleAdvanced healthcare materials2024

Transcriptomic Analysis Reveals the Heterogeneous Role of Conducting Films Upon Electrical Stimulation.

Nicholas B Lawler, Uditi Bhatt, Vipul Agarwal, Cameron W Evans, Priya Kaluskar, Sebastian E Amos, Kai Chen, Yin Yao, Haibo Jiang, Yu Suk Choi and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced healthcare materials, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Nicholas B LawlerSchool of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.ORCID 0000-0002-8302-5371
Uditi BhattSchool of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.ORCID 0000-0003-4188-0457
Vipul AgarwalCluster for Advanced Macromolecular Design (CAMD), School of Chemical Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.ORCID 0000-0002-6239-5410
Cameron W EvansSchool of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.ORCID 0000-0003-2312-9803
Priya KaluskarSchool of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.ORCID 0000-0001-7414-3635
Sebastian E AmosSchool of Human Sciences, The University of Western Australia, Perth, WA, 6009, Australia.
Kai ChenSchool of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.
Yin YaoElectron Microscope Unit, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, NSW, 2052, Australia.
Haibo JiangSchool of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.
Yu Suk ChoiARC Training Centre for Next-Gen Technologies in Biomedical Analysis, School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.ORCID 0000-0002-0241-0447
Minghao ZhengPerron Institute for Neurological and Translational Science, Perth, WA, 6009, Australia.ORCID 0000-0003-1185-4768
Dino SpagnoliSchool of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.ORCID 0000-0001-6367-4748
Irene Suarez-MartinezDepartment of Physics and Astronomy, Curtin University, Perth, WA, 6102, Australia.
Per B ZetterlundCluster for Advanced Macromolecular Design (CAMD), School of Chemical Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.ORCID 0000-0003-3149-4464
Vincent P WallaceSchool of Physics, Mathematics and Computing, The University of Western Australia, Perth, WA, 6009, Australia.ORCID 0000-0003-3814-5400
Alan R HarveyPerron Institute for Neurological and Translational Science, Perth, WA, 6009, Australia.ORCID 0000-0002-2590-831X
Stuart I HodgettsPerron Institute for Neurological and Translational Science, Perth, WA, 6009, Australia.ORCID 0000-0002-3318-0410
K Swaminathan IyerSchool of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.ORCID 0000-0001-9329-4930

Funding

Australian Research Council DP190100831Australian Research Council FT180100683Neurotrauma Research Program of Western Australia
6 · The paper itself

Abstract

Central nervous system (CNS) injuries and neurodegenerative diseases have markedly poor prognoses and can result in permanent dysfunction due to the general inability of CNS neurons to regenerate. Differentiation of transplanted stem cells has emerged as a therapeutic avenue to regenerate tissue architecture in damaged areas. Electrical stimulation is a promising approach for directing the differentiation outcomes and pattern of outgrowth of transplanted stem cells, however traditional inorganic bio-electrodes can induce adverse effects such as inflammation. This study demonstrates the implementation of two organic thin films, a polymer/reduced graphene oxide nanocomposite (P(rGO)) and PEDOT:PSS, that have favorable properties for implementation as conductive materials for electrical stimulation, as well as an inorganic indium tin oxide (ITO) conductive film. Transcriptomic analysis reveals that electrical stimulation improves neuronal differentiation of SH-SY5Y cells on all three films, with the greatest effect for P(rGO). Unique material- and electrical stimuli-mediated effects are observed, associated with differentiation, cell-substrate adhesion, and translation. The work demonstrates that P(rGO) and PEDOT:PSS are highly promising organic materials for the development of biocompatible, conductive scaffolds that will enhance electrically-aided stem cell therapeutics for CNS injuries and neurodegenerative diseases.

Indexed as

Cell DifferentiationElectric StimulationGraphiteBiocompatible MaterialsBridged Bicyclo Compounds, HeterocyclicCell AdhesionCell Line, TumorElectric ConductivityGene Expression ProfilingHumansNanocompositesNeuronsPolymersTin CompoundsTranscriptomeBiocompatible MaterialsBridged Bicyclo Compounds, Heterocyclicgraphene oxideGraphiteindium tin oxidepoly(3,4-ethylene dioxythiophene)PolymersTin Compoundselectrical stimulationITOneuronal differentiationPEDOT:PSSreduced graphene oxideregenerative therapyrGO

Identifiers

PMID39221662
PMCPMC11670273

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.