Evidence map›Paper›PMID 39220701›Full record

ArticleACS central science2024

Protein Complex Heterogeneity and Topology Revealed by Electron Capture Charge Reduction and Surface Induced Dissociation.

Jared B Shaw, Sophie R Harvey, Chen Du, Zhixin Xu, Regina M Edgington, Eduardo Olmedillas, Erica Ollmann Saphire, Vicki H Wysocki

Abstract read
In one paragraph

Article in ACS central science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Article
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  7. Top-Down and Middle-Down Mass Spectrometry of Antibodies.Molecular & cellular proteomics : MCP · 2025
    Review
  8. Article
  9. Review
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  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Design of a yeast SUMO tag to eliminate internal translation initiation.Protein science : a publication of the Protein Society · 2025
    Article
  17. AsymmetricJournal of the American Chemical Society · 2024
    Article
  18. Article
  19. To 200,000ACS central science · 2024
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Jared B ShawDepartment of Chemistry, University of Nebraska, Lincoln, Nebraska 68588, United States.
Sophie R HarveyNative Mass Spectrometry Guided Structural Biology Center, Ohio State University, Columbus, Ohio 43210, United States.ORCID https://orcid.org/0000-0003-0763-8173
Chen DuNative Mass Spectrometry Guided Structural Biology Center, Ohio State University, Columbus, Ohio 43210, United States.
Zhixin XuNative Mass Spectrometry Guided Structural Biology Center, Ohio State University, Columbus, Ohio 43210, United States.
Regina M EdgingtonDepartment of Chemistry and Biochemistry, Ohio State University, Columbus, Ohio 43210, United States.
Eduardo OlmedillasCenter for Vaccine Innovation, La Jolla Institute for Immunology, La Jolla, California 92037, United States.
Erica Ollmann SaphireCenter for Vaccine Innovation, La Jolla Institute for Immunology, La Jolla, California 92037, United States.
Vicki H WysockiNative Mass Spectrometry Guided Structural Biology Center, Ohio State University, Columbus, Ohio 43210, United States.ORCID https://orcid.org/0000-0003-0495-2538

Funding

Targeted mass spectrometry approaches to understand CART processing and recepter interactionsP20GM113126 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI ZHOU, YOU · 2016 to 2025
$20.8M
Technology and Computational CoreP01AI165072 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI WESEMANN, DUANE R. · 2021 to 2023
$19.6M
Waters Select Series Cyclic IMS for P41 Native MS Resource Application to Alzheimer's DiseaseP41GM128577 · NIGMS · OHIO STATE UNIVERSITY · PI WYSOCKI, VICKI H. · 2018 to 2022
$7.6M
Native Mass Spectrometry Guided Structural Biology CenterRM1GM149374 · NIGMS · OHIO STATE UNIVERSITY · PI Vicki H. Wysocki · 2023 to 2026
$5.0M
Advancing native top-down protein analysis with hybrid SID/ECD technologyR43GM140749 · NIGMS · E-MSION, INC. · PI SHAW, JARED BRYAN · 2021 to 2021
$252k
NIAID NIH HHS P01 AI165072NIGMS NIH HHS P20 GM113126NIGMS NIH HHS P41 GM128577NIGMS NIH HHS R43 GM140749NIGMS NIH HHS RM1 GM149374
6 · The paper itself

Abstract

We illustrate the utility of native mass spectrometry (nMS) combined with a fast, tunable gas-phase charge reduction, electron capture charge reduction (ECCR), for the characterization of protein complex topology and glycoprotein heterogeneity. ECCR efficiently reduces the charge states of tetradecameric GroEL, illustrating Orbitrap

Identifiers

PMID39220701
PMCPMC11363329

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.