Evidence map›Paper›PMID 39218837›Full record

ArticleReproductive sciences (Thousand Oaks, Calif.)2025

ZEB1 Promotes Epithelial-Mesenchymal Transition of Endometrial Epithelial Cells and Plays a Critical Role in Embryo Implantation in Mice.

Zhong Xu, Huan-Huan Yang, Hou-Zhi Chen, Bi-Zhen Huang, Ming Yang, Zhen-Hua Liao, Bi-Qing Xiao, Hong-Qin Chen, Jing Ran

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Loss of Function of theCurrent issues in molecular biology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zhong XuThe First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, 55 Zhenhai Road, Xiamen, 361003, PR China.
Huan-Huan YangThe First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, 55 Zhenhai Road, Xiamen, 361003, PR China.
Hou-Zhi ChenDuke Kunshan University, Duke University, Durham, NC, 27708, USA.
Bi-Zhen HuangThe First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, 55 Zhenhai Road, Xiamen, 361003, PR China.
Ming YangThe First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, 55 Zhenhai Road, Xiamen, 361003, PR China.
Zhen-Hua LiaoThe First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, 55 Zhenhai Road, Xiamen, 361003, PR China.
Bi-Qing XiaoSchool of Clinical Medicine, Fujian Medical University, Fuzhou, 350001, Fujian, PR China.
Hong-Qin ChenSchool of Clinical Medicine, Fujian Medical University, Fuzhou, 350001, Fujian, PR China.
Jing RanThe First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, 55 Zhenhai Road, Xiamen, 361003, PR China. ranj@xmu.edu.cn.ORCID 0000-0002-8529-3441

Funding

Fujian Provincial Health Science and Technology Plan Project for Young and Middle-aged Backbone Talent Training Project 2023GGB02 to JRNational Natural Science Foundation of China 81601284 to JR
6 · The paper itself

Abstract

Zinc finger E-box binding homeobox 1 (ZEB1) promotes epithelial-mesenchymal transition (EMT) in carcinogenesis, but its role in embryo implantation has not yet been well studied. In the present study we evaluated the hypothesis that ZEB1-induced EMT is essential for embryo implantation in vivo. Endometrial epithelium from female Kunming mice (non-pregnant, and pregnant from day 2.5 to 6.5) were collected for assessment of mRNA/protein expression of ZEB1, and EMT markers E-cadherin and vimentin, by employment of real-time quantitative reverse transcription PCR, Western blot, and immunohistochemical staining. To test if knockdown of ZEB1 affects embryo implantation in vivo, mice received intrauterine injection of shZEB1 before the number of embryos implanted was counted. The results showed that, ZEB1 was highly expressed at both mRNA and protein levels in the mouse endometrium on day 4.5 of pregnancy, paralleled with down-regulated E-cadherin and up-regulated vimentin expression (P < 0.05). Intrauterine injection of shZEB1 markedly suppressed embryo implantation in mice (P < 0.01). Conclusively, the present work demonstrated that ZEB1 is essential for embryo implantation under in vivo condition, and is possibly due to its effect on modulation of endometrial receptivity through EMT.

Indexed as

Embryo ImplantationEndometriumEpithelial CellsEpithelial-Mesenchymal TransitionZinc Finger E-box-Binding Homeobox 1AnimalsCadherinsFemaleMicePregnancyVimentinCadherinsVimentinZEB1 protein, mouseZinc Finger E-box-Binding Homeobox 1E-cadherinEmbryo implantationEndometrial receptivityEpithelial–mesenchymal transitionVimentinZinc finger E-box binding homeobox 1

Identifiers

PMID39218837
PMCPMC11978684

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.