Evidence map›Paper›PMID 39215885›Full record

ArticleMarine biotechnology (New York, N.Y.)2024

Development of Reliable Male-Specific Molecular Markers for Genetic Sex Identification in Sea Cucumber Apostichopus japonicus.

Xinghai Liu, Lina Sun, Ziming Li, Hongyuan Zhao, Yujia Yang

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Article in Marine biotechnology (New York, N.Y.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Xinghai LiuThe Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, China.
Lina SunCAS Key Laboratory of Marine Ecology and Environmental Sciences & Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
Ziming LiThe Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, China.
Hongyuan ZhaoCAS Key Laboratory of Marine Ecology and Environmental Sciences & Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
Yujia YangThe Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, China. yangyujia@ouc.edu.cn.

Funding

National Key Research and Development Program of China 2022YFD2401305National Natural Science Foundation of China 42206084
6 · The paper itself

Abstract

Apostichopus japonicus is an important marine aquaculture species in China, with high nutritional and economic value. In A. japonicus, there is no obvious sexual dimorphism in external appearance, and sex differentiation primarily relies on the observation of mature gonads after dissection, which leads to difficulties in sex identification. The confusion in sex identification greatly reduces breeding efficiency in the sea cucumber industry. Therefore, developing a reliable sex-specific marker is crucial. In this study, we identified 586 male-specific sequences through whole-genome assembly and sequence alignment, but did not identify any female-specific sequences, inferring an XY-type sex determination system in sea cucumbers. We developed a set of male-specific molecular markers to establish an accurate, stable, and widely adaptable genetic sex identification technique for A. japonicus. The male-specific molecular markers were validated with 100% accuracy in sea cucumber populations from six different geographical regions in China. In conclusion, this study provides further evidence for the XY-type sex determination system in A. japonicus and establishes an effective genetic sex identification method for multi-geographic populations, which benefits future study on reproductive biology and has significant implications in sea cucumber aquaculture industry.

Indexed as

StichopusAnimalsAquacultureChinaFemaleGenetic MarkersGonadsMaleSea CucumbersSex Determination AnalysisSex Determination ProcessesGenetic MarkersApostichopus japonicusGenetic sex identificationMolecular markersSea cucumber breedingSex-specific markers

Identifiers

PMID39215885

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