ArticlePlant & cell physiology2024
Heritable Tissue-Culture-Free Gene Editing in Nicotiana benthamiana through Viral Delivery of SpCas9 and sgRNA.
Article in Plant & cell physiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Behind (cell) walls.Nature biotechnology · 2026Article
- Turnip Mosaic Virus-Based gRNA Delivery System for Plant Genome Editing.Plant direct · 2026Article
- Evolution and implementation of EU regulation on new genomic techniques.Nature plants · 2026Article
- A Modified Cas9 Scaffold Allows Extension of the Virus-Induced Gene Editing Technology to the Large Potyvirus Genus.Plant biotechnology journal · 2026Article
- Genome Editing Approaches in Flax (International journal of molecular sciences · 2026Review
- Developing a mobile RNA delivery system for grafting trait improvement.EMBO reports · 2026Article
- Virus-induced transgene- and tissue culture-free heritable genome editing in tomato.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Plant viruses as next-generation vectors for transgene-free genome editing, gene regulation, and rapid crop improvement.Plant molecular biology · 2026Review
- Virus-induced genome editing enables functional genomics across diverse plant species.Molecules and cells · 2026Review
- Virus-Mediated System for Simultaneous Gene Silencing and Genome Editing in Cotton.Plants (Basel, Switzerland) · 2026Article
- Virus induced gene editing using potyviral vectors in Cas12a expressing plants.Horticulture research · 2026Article
- Host ESCRT machinery orchestrates the assembly of tomato spotted wilt virus ribonucleoproteins.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Quinoa as a naturally stress-resistant crop: current status and future promises.Stress biology · 2026Review
- Application of compact CRISPR/Cas nucleases for citrus genome editing.Transgenic research · 2026Article
- Next-generation CRISPR systems and advanced delivery paradigms for precision crop improvement: a comparative case study in sugar beet (Frontiers in plant science · 2026Review
- Genetic transformation and genome editing of flax (Frontiers in plant science · 2026Review
- Heritable Tissue-Culture-Free Gene Editing in Nicotiana benthamiana Using a Meristem-Invading Virus Vector.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Host Factors Promoting the LTR Retrotransposon Life Cycle in Plant Cells: Current Knowledge and Future Directions.International journal of molecular sciences · 2025Review
- Positive-Strand RNA Viruses Induce LTR Retrotransposon Transcription and Extrachromosomal Circular DNA Generation in Plants.International journal of molecular sciences · 2025Article
- Knockout of SSIIa in waxy barley blocks starch biosynthesis and increases soluble sugar and Fructan accumulation.Functional & integrative genomics · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Conventional plant gene editing requires laborious tissue-culture-mediated transformation, which restricts the range of applicable plant species. In this study, we developed a heritable and tissue-culture-free gene editing method in Nicotiana benthamiana using tobacco ringspot virus (TRSV) as a vector for in planta delivery of Cas9 and single-guide RNA (sgRNA) to shoot apical meristems. Agrobacterium-mediated inoculation of the TRSV vector induced systemic and heritable gene editing in Nicotiana benthamiana PHYTOENE DESATURASE. Transient downregulation of RNA silencing enhanced gene editing efficiency, resulting in an order of magnitude increase (0.8-13.2%) in the frequency of transgenerational gene editing. While the TRSV system had a preference for certain sgRNA sequences, co-inoculation of a TRSV vector carrying only Cas9 and a tobacco rattle virus vector carrying sgRNA successfully introduced systemic mutations with all five tested sgRNAs. Extensively gene-edited lateral shoots occasionally grew from plants inoculated with the virus vectors, the transgenerational gene editing frequency of which ranged up to 100%. This virus-mediated heritable gene editing method makes plant gene editing easy, requiring only the inoculation of non-transgenic plants with a virus vector(s) to obtain gene-edited individuals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.