Evidence map›Paper›PMID 39215297›Full record

ArticleMalaria journal2024

Comparison of three rapid diagnostic tests for Plasmodium falciparum diagnosis in Ghana.

Tolulope Adeyemi Kayode, Agyapong Kofi Addo, Thomas Kwame Addison, Austine Tweneboah, Stephen Opoku Afriyie, Dawood Ackom Abbas, Ayesha Seth, Abraham K Badu-Tawiah, Kingsley Badu, Cristian Koepfli

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Malaria journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Tolulope Adeyemi KayodeEck Institute for Global Health and Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA.
Agyapong Kofi AddoDepartment of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Thomas Kwame AddisonDepartment of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Austine TweneboahDepartment of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Stephen Opoku AfriyieDepartment of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Dawood Ackom AbbasDepartment of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Ayesha SethDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA.
Abraham K Badu-TawiahDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA.
Kingsley BaduDepartment of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Cristian KoepfliEck Institute for Global Health and Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA. ckoepfli@nd.edu.

Funding

Malaria Management through an On-demand Diagnostic Approach using Novel Ionic ProbesR01AI143809 · NIAID · OHIO STATE UNIVERSITY · PI BADU-TAWIAH, ABRAHAM · 2019 to 2023
$1.3M
NIAID NIH HHS R01 AI143809NIH HHS R01-AI-143809
6 · The paper itself

Abstract

backgroundAccurate diagnosis and timely treatment are crucial in combating malaria.

methodsA total of 449 samples were screened for Plasmodium falciparum infection by expert microscopy, qPCR, and three RDTs, namely Rapigen Biocredit Malaria Ag Pf (detecting HRP2 and pLDH on separate bands), Abbott NxTek Eliminate Malaria Ag Pf (detecting HRP2), and SD Bioline Malaria Ag Pf (detecting HRP2). hrp2/3 deletion typing was done by digital PCR.

results45.7% (205/449) individuals tested positive by qPCR for P. falciparum with a mean parasite density of 12.5 parasites/μL. Using qPCR as reference, the sensitivity of microscopy was 28.3% (58/205), the Biocredit RDT was 52.2% (107/205), the NxTek RDT was 49.3% (101/205), and the Bioline RDT was 39.5% (81/205). When only samples with densities > 20 parasites/μL were included (n = 89), sensitivity of 62.9% (56/89) by microscopy, 88.8% (79/89) by Biocredit, 88.8% (79/89) by NxTek, and 78.7% (70/89) by Bioline were obtained. All three RDTs demonstrated specificities > 95%. The limits of detection (95% probability that a sample tested positive) was 4393 parasites/μL (microscopy), 56 parasites/μL (Biocredit, considering either HRP2 or pLDH), 84 parasites/μL (NxTek), and 331 parasites/μL (Bioline). None of the three qPCR-confirmed P. falciparum positive samples, identified solely through the pLDH target, or eight samples negative for all RDTs but qPCR-positive at densities > 20 parasites/µL carried hrp2/3 deletions.

conclusionThe Biocredit and NxTek RDTs demonstrated comparable diagnostic efficacies. All three RDTs performed better than microscopy.

Indexed as

Diagnostic Tests, RoutineMalaria, FalciparumPlasmodium falciparumSensitivity and SpecificityAdolescentAdultAgedAged, 80 and overChildChild, PreschoolFemaleGhanaHumansInfantMaleMicroscopyHRP2Malaria diagnosisPLDHRapid diagnostic testUltrasensitive RDT

Identifiers

PMID39215297
PMCPMC11363606

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.