Evidence map›Paper›PMID 39212886›Full record

ArticleDoklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections2024

Generation of LEPR Knockout Rabbits with CRISPR/CAS9 System.

Yu Yu Silaeva, P D Safonova, D V Popov, M A Filatov, Yu D Okulova, R A Shafei, O I Skobel, D E Vysotskii, Yu D Gubarev, V I Glazko and 4 more

Abstract read
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Article in Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yu Yu SilaevaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
P D SafonovaInstitute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
D V PopovAfanas'ev Institute of Fur-bearing Animal Breeding and Rabbit Breeding, Rodniki, Moscow Region, Russia.
M A FilatovCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
Yu D OkulovaCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
R A ShafeiMoscow State University, Moscow, Russia.
O I SkobelAfanas'ev Institute of Fur-bearing Animal Breeding and Rabbit Breeding, Rodniki, Moscow Region, Russia.
D E VysotskiiAfanas'ev Institute of Fur-bearing Animal Breeding and Rabbit Breeding, Rodniki, Moscow Region, Russia.
Yu D GubarevBelgorod State National Research University, Belgorod, Russia.
V I GlazkoAfanas'ev Institute of Fur-bearing Animal Breeding and Rabbit Breeding, Rodniki, Moscow Region, Russia.
T T GlazkoAfanas'ev Institute of Fur-bearing Animal Breeding and Rabbit Breeding, Rodniki, Moscow Region, Russia.
P G GeorgievInstitute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
G Yu KosovskyAfanas'ev Institute of Fur-bearing Animal Breeding and Rabbit Breeding, Rodniki, Moscow Region, Russia.
M V ShepelevCenter for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia. mshepelev@mail.ru.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The LEPR gene encodes a leptin hormone receptor, and its mutations are associated with morbid obesity, dysregulation of lipid metabolism, and fertility defects in humans. Spontaneous Lepr mutations have been described in rodents, and Lepr knockout animals have been generated, in particular, using the CRISPR/Cas9 system. Lipid metabolism in rodents significantly differs from that in humans or rabbits, and rabbits are therefore considered as the most relevant model of morbid obesity and lipid metabolism dysregulation in humans. LEPR knockout rabbits have not been reported so far. In this work a LEPR knockout rabbit was generated by introducing a deletion of the region around LEPR exon 10 using the CRISPR/Cas9 system. The body weight of the knockout rabbit was significantly higher than the average body weight of the wild type rabbits. CRISPR/Cas9-mediated generation of LEPR knockout rabbits will allow the development of a model of morbid obesity and endocrine defects due to leptin receptor mutations in humans.

Indexed as

CRISPR-Cas SystemsGene Knockout TechniquesReceptors, LeptinAnimalsRabbitsReceptors, LeptinCRISPR/Cas9genetically modified rabbitLEPRleptin

Identifiers

PMID39212886

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.