Effect of the estrous cycle on zinc transporter expression in bovine cumulus-oocyte complexes and oviduct epithelial cells.

A M Pascua, G Barbisan, N Nikoloff, A C Carranza-Martín, M C Fabra, J P Anchordoquy, M Balbi, C Furnus, J M Anchordoquy

Abstract read

In one paragraph

Article in Veterinary research communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

9 authors.

A M PascuaFacultad de Ciencias Veterinarias UNLP, IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N Dulout" (UNLP-CONICET LA PLATA), Calles 60 y 118, B1904AMA, La Plata, Buenos Aires, Argentina.

G BarbisanFacultad de Ciencias Veterinarias UNLP, IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N Dulout" (UNLP-CONICET LA PLATA), Calles 60 y 118, B1904AMA, La Plata, Buenos Aires, Argentina.

N NikoloffFacultad de Ciencias Veterinarias UNLP, IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N Dulout" (UNLP-CONICET LA PLATA), Calles 60 y 118, B1904AMA, La Plata, Buenos Aires, Argentina.

A C Carranza-MartínFacultad de Ciencias Veterinarias UNLP, IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N Dulout" (UNLP-CONICET LA PLATA), Calles 60 y 118, B1904AMA, La Plata, Buenos Aires, Argentina.

M C FabraFacultad de Ciencias Veterinarias UNLP, IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N Dulout" (UNLP-CONICET LA PLATA), Calles 60 y 118, B1904AMA, La Plata, Buenos Aires, Argentina.

J P AnchordoquyFacultad de Ciencias Veterinarias UNLP, IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N Dulout" (UNLP-CONICET LA PLATA), Calles 60 y 118, B1904AMA, La Plata, Buenos Aires, Argentina.

M BalbiFacultad de Ciencias Veterinarias UNLP, IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N Dulout" (UNLP-CONICET LA PLATA), Calles 60 y 118, B1904AMA, La Plata, Buenos Aires, Argentina.

C FurnusFacultad de Ciencias Veterinarias UNLP, IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N Dulout" (UNLP-CONICET LA PLATA), Calles 60 y 118, B1904AMA, La Plata, Buenos Aires, Argentina.

J M AnchordoquyFacultad de Ciencias Veterinarias UNLP, IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N Dulout" (UNLP-CONICET LA PLATA), Calles 60 y 118, B1904AMA, La Plata, Buenos Aires, Argentina. mateoanchordoquy@fcv.unlp.edu.ar.

Funding

Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación PICT 2020-0819Consejo Nacional de Investigaciones Científicas y Técnicas PIP 2021-2839

6 · The paper itself

Abstract

During the luteal and follicular phases of the estrous cycle, cumulus-oocyte complexes (COC) and oviduct epithelial cells (OEC) undergo notable physiological and morphological changes. Maintaining proper zinc (Zn) homeostasis is crucial in both somatic and germinal mammalian cells. This study aimed to assess the impact of the estrous phase (luteal or follicular) on Zn transporter expression in bovine COC and OEC (BOEC). The expression of Zn transporters Slc39a6 (ZIP6), Slc39a8 (ZIP8), Slc39a14 (ZIP14), Slc30a3 (ZnT3), Slc30a7 (ZnT7), and Slc30a9 (ZnT9) was analyzed in COC and BOEC from cows during the luteal or follicular phases. Gene expression of ZIP6, ZIP14, and ZnT9 was quantified in COC and BOEC. The gene expression in the remaining transporters could not be quantified due to low mRNA levels (ZIP8 and ZnT3 in COC and BOEC; ZnT7 in BOEC) or absence of expression (ZnT7 in COC). In COC, the relative expression (RE) of all three transporters was higher in the luteal phase compared to the follicular phase (P ≤ 0.05). In BOEC, the luteal phase increased the RE of ZIP 6 (P ≤ 0.05), decreased the RE of ZnT9 (P ≤ 0.05), and did not modify the RE of ZIP14 (P > 0.05) compared to the follicular phase. In conclusion, the study reveals differences in the gene expression of ZIP6, ZIP14, and ZnT9 according to the estrous cycle phase in ex vivo samples of bovine COC and OEC.

Indexed as

Cumulus CellsEpithelial CellsEstrous CycleOocytesAnimalsCarrier ProteinsCation Transport ProteinsCattleFallopian TubesFemaleGene Expression RegulationOviductsCarrier ProteinsCation Transport Proteinszinc-binding proteinCattleFollicular phaseLuteal phaseOviductZn transporter

Identifiers

PMID39212812

DOI10.1007/s11259-024-10523-9

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.