Evidence map›Paper›PMID 39211933›Full record

ArticleHeliyon2024

Rapid and robust isolation of microglia and vascular cells from brain subregions for integrative single-cell analyses.

Efthalia Preka, Alejandro Lastra Romero, Ying Sun, Yara Onetti Vilalta, Thea Seitz, Adamantia Fragkopoulou, Christer Betsholtz, Ahmed M Osman, Klas Blomgren

Abstract read
In one paragraph

Article in Heliyon, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Isolation of Primary Brain Cells: Challenges and Solutions.Archives of clinical and biomedical research · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Efthalia PrekaDepartment of Women's and Children's Health, Biomedicum A4, Karolinska Institutet, 171 77, Stockholm, Sweden.
Alejandro Lastra RomeroDepartment of Women's and Children's Health, Biomedicum A4, Karolinska Institutet, 171 77, Stockholm, Sweden.
Ying SunDepartment of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Sweden.
Yara Onetti VilaltaDepartment of Women's and Children's Health, Biomedicum A4, Karolinska Institutet, 171 77, Stockholm, Sweden.
Thea SeitzDepartment of Women's and Children's Health, Biomedicum A4, Karolinska Institutet, 171 77, Stockholm, Sweden.
Adamantia FragkopoulouDepartment of Women's and Children's Health, Biomedicum A4, Karolinska Institutet, 171 77, Stockholm, Sweden.
Christer BetsholtzDepartment of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Sweden.
Ahmed M OsmanDepartment of Women's and Children's Health, Biomedicum A4, Karolinska Institutet, 171 77, Stockholm, Sweden.
Klas BlomgrenDepartment of Women's and Children's Health, Biomedicum A4, Karolinska Institutet, 171 77, Stockholm, Sweden.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell isolation protocols from brain tissue include prolonged

Indexed as

Endothelial cellsNeuroinflammationPericytesRNA sequencingVascular disease

Identifiers

PMID39211933
PMCPMC11357767

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.