Evidence map›Paper›PMID 39210951›Full record

ArticleBio-protocol2024

Using Localization Microscopy to Quantify Calcium Channels at Presynaptic Boutons.

Brian D Mueller, Sean A Merrill, Lexy Von Diezmann, Erik M Jorgensen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Brian D MuellerSchool of Biological Sciences, University of Utah, Salt Lake City, UT, USA.
Sean A MerrillSchool of Biological Sciences, University of Utah, Salt Lake City, UT, USA.
Lexy Von DiezmannDept. of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN, USA.
Erik M JorgensenSchool of Biological Sciences, University of Utah, Salt Lake City, UT, USA.

Funding

SYNAPTIC FUNCTION IN THE NEMATODE C. ELEGANSR01NS034307 · NINDS · UNIVERSITY OF UTAH · PI JORGENSEN, ERIK M · 1995 to 2024
$5.8M
Synaptic Calcium ChannelsF31NS084826 · NINDS · UNIVERSITY OF UTAH · PI MERRILL, SEAN ARIC · 2013 to 2015
$86k
NINDS NIH HHS F31 NS084826NINDS NIH HHS R01 NS034307
6 · The paper itself

Abstract

Calcium channels at synaptic boutons are critical for synaptic function, but their number and distribution are poorly understood. This gap in knowledge is primarily due to the resolution limits of fluorescence microscopy. In the last decade, the diffraction limit of light was surpassed, and fluorescent molecules can now be localized with nanometer precision. Concurrently, new gene editing strategies allowed direct tagging of the endogenous calcium channel genes-expressed in the correct cells and at physiological levels. Further, the repurposing of self-labeling enzymes to attach fluorescent dyes to proteins improved photon yields enabling efficient localization of single molecules. Here, we describe tagging strategies, localization microscopy, and data analysis for calcium channel localization. In this case, we are imaging calcium channels fused with SNAP or HALO tags in live anesthetized

Indexed as

Calcium channelsC. elegansNeurosciencePre-synapseQuantitative localization microscopySuper-resolution microscopy

Identifiers

PMID39210951
PMCPMC11349493

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.