Isolation and Identification of Lactic Acid Bacteria from Environmental Samples.

Akihito Endo, Yasuhiro Tanizawa, Shintaro Maeno, Masanori Arita

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Low-Protein-Fed Chickens Benefit from ProbioticAnimals : an open access journal from MDPI · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Akihito EndoDepartment of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan. a3endou@nodai.ac.jp.

Yasuhiro TanizawaCenter for Information Biology, National Institute of Genetics, Shizuoka, Japan.

Shintaro MaenoResearch Center for Advanced Science and Innovation, Organization for Research Initiatives, Yamaguchi University, Yamaguchi, Japan.

Masanori AritaCenter for Information Biology, National Institute of Genetics, Shizuoka, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Isolation is the first and crucial step to study possible roles of lactic acid bacteria (LAB) in environment, especially in food fermentation. It is also important to use the organisms for further application. LABs are diverse bacterial group and have diverse growth characteristics. Culture condition of LAB is thus varied, and selection of a suitable culture medium is essential for the purposes. Identification is also important step, since certain desirable and undesirable characteristics are shared within species. Identification was classically carried out by phenotypic characteristics but is usually performed for DNA sequence-based approaches. 16S rRNA gene sequencing is generally used for the identification, and sequencing of housekeeping genes is used when needed. In addition, identification based on whole-genome sequence similarities was well established during the last decade. Here, we describe methods for isolation and identification of LAB briefly.

Indexed as

LactobacillalesRNA, Ribosomal, 16SCulture MediaDNA, BacterialEnvironmental MicrobiologySequence Analysis, DNACulture MediaDNA, BacterialRNA, Ribosomal, 16S16S rRNA geneCulture mediumHousekeeping genesIdentificationIsolationLactic acid bacteriaWhole-genome sequence similarities

Identifiers

PMID39210167

DOI10.1093/nar/gkt1226

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.