ReviewNature protocols2024
Tutorial: fluorescence lifetime microscopy of membrane mechanosensitive Flipper probes.
Review in Nature protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Engineering migrasome-inspired extracellular vesicles through temperature stimuli on thermoresponsive substrate for retraction fiber formation and hypoosmotic vesiculation.Materials today. Bio · 2026Article
- Red-to-Near-Infrared Fluorescence Resonance Energy Transfer Reporters for Dual Imaging Applications.Small methods · 2026Article
- Apical-out polarity in epithelial spheroids requires α6β4 integrins, cell proliferation and anchorage independence.Journal of cell science · 2026Article
- Direct Observation and Measurement of Membrane Tension in Xenopus laevis Animal Cap Explants Using the Mechanosensitive Dye, Flipper-TR.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy.Communications biology · 2025Article
- Adherent cells sustain membrane tension gradients independently of migration.Nature communications · 2025Article
- Nanoscale Analysis beyond Imaging by Atomic Force Microscopy: Molecular Perspectives on Oncology and Neurodegeneration.Small science · 2025Review
- Exploring bacteria-surface interactions with a fluorescent membrane tension probe.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Capturing nematic order on tissue surfaces of arbitrary geometry.Nature communications · 2025Article
- Advanced optical microscopy methods forBiophysical reviews · 2025Review
- A gentle palette of plasma membrane dyes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.Biophotonics discovery · 2025Review
- Peeking into the future: inferring mechanics in dynamical tissues.Biochemical Society transactions · 2024Review
- Tensing Flipper: Photosensitized Manipulation of Membrane Tension, Lipid Phase Separation, and Raft Protein Sorting in Biological Membranes.Journal of the American Chemical Society · 2024Article
- Fluorescence lifetime imaging microscopy of flexible and rigid dyes probes the biophysical properties of synthetic and biological membranes.Biophysical journal · 2024Article
- Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding.Advanced drug delivery reviews · 2023Review
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
Measuring forces within living cells remains a technical challenge. In this Tutorial, we cover the development of hydrophobic mechanosensing fluorescent probes called Flippers, whose fluorescence lifetime depends on lipid packing. Flipper probes can therefore be used as reporters for membrane tension via the measurement of changes in their fluorescence lifetime. We describe the technical optimization of the probe for imaging and provide working examples for their characterizations in a variety of biological and in vitro systems. We further provide a guideline to measure biophysical parameters of cellular membranes by fluorescence lifetime imaging microscopy using Flipper probes, providing evidence that flippers can report long range forces in cells, tissues and organs.
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39210094What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.