ArticleNature biomedical engineering2024
Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples.
Article in Nature biomedical engineering, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Organoid Intelligent Morphomics: Decoding the organoid morphome through artificial intelligence from phenotypic quantification to mechanistic insight.Bioactive materials · 2027Review
- Adapting upright light sheet fluorescence microscopy for imaging at air-liquid interface.Npj imaging · 2026Article
- Affordable centimeter-scale 3D microscopy with submicrometer resolution.Nature biotechnology · 2026Article
- Hybrid solid-liquid optics enable scalable, high-resolution light-sheet microscopy across diverse immersion media.Nature biotechnology · 2026Article
- Adapting Upright Light Sheet Fluorescence Microscopy for Imaging at Air-Liquid Interface.bioRxiv : the preprint server for biology · 2026Article
- Technological innovations and high-throughput applications of light-sheet microscopy.Biophysics reports · 2026Article
- Article
- Advanced imaging strategies in cardiac organoids: bridging the gap between structural complexity and functional analysis.Cardiovascular diabetology · 2026Review
- Multiscale 3D Whole Joint Cellular and Molecular Mapping Reveals Disease-Specific Neurovascular Plasticity Underlying the Structure-Pain Relationship.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue.Nature biotechnology · 2025Article
- Altair-LSFM A High-Resolution, Easy-to-Build Light-Sheet Microscope for Sub-Cellular Imaging.bioRxiv : the preprint server for biology · 2025Article
- Hybrid Solid-Liquid Optics Enable Scalable, High-Resolution, Multi-Immersion Light-Sheet Microscopy.bioRxiv : the preprint server for biology · 2025Article
- Advancing Multicolor Super-Resolution Volume Imaging: Illuminating Complex Cellular Dynamics.JACS Au · 2025Review
- Brain-wide immunolabeling and tissue clearing applications for engram research.Neurobiology of learning and memory · 2025Review
- Classification of psychedelics and psychoactive drugs based on brain-wide imaging of cellular c-Fos expression.Nature communications · 2025Article
- Machine learning methods for histopathological image analysis: Updates in 2024.Computational and structural biotechnology journal · 2025Review
- Classification of psychedelics and psychoactive drugs based on brain-wide imaging of cellular c-Fos expression.bioRxiv : the preprint server for biology · 2024Article
- Cellular arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms.PLoS biology · 2024Article
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15 authors.
Funding
Abstract
Light-sheet fluorescence microscopy (LSFM) is a widely used technique for imaging cleared tissue and living samples. However, high-performance LSFM systems are typically expensive and not easily scalable. Here we introduce a low-cost, scalable and versatile LSFM framework, which we named 'projected light-sheet microscopy' (pLSM), with high imaging performance and small device and computational footprints. We characterized the capabilities of pLSM, which repurposes readily available consumer-grade components, optimized optics, over-network control architecture and software-driven light-sheet modulation, by performing high-resolution mapping of cleared mouse brains and of post-mortem pathological human brain samples, and via the molecular phenotyping of brain and blood-vessel organoids derived from human induced pluripotent stem cells. We also report a method that leverages pLSM for the live imaging of the dynamics of sparsely labelled multi-layered bacterial pellicle biofilms at an air-liquid interface. pLSM can make high-resolution LSFM for biomedical applications more accessible, affordable and scalable.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.