Evidence map›Paper›PMID 39209550›Full record

ArticleDrug metabolism and disposition: the biological fate of chemicals2024

Molecular Mechanisms for the Selective Transport of Dichlorofluorescein by Human Organic Anion Transporting Polypeptide 1B1.

Han Liu, Lanjing Li, Ting Liang, Ru Huan, Bruno Hagenbuch, Chunshan Gui

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Article in Drug metabolism and disposition: the biological fate of chemicals, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Han LiuCollege of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, China (H.L., L.L., T.L., R.H., C.G.); and Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, Kansas (B.H.).
Lanjing LiCollege of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, China (H.L., L.L., T.L., R.H., C.G.); and Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, Kansas (B.H.).
Ting LiangCollege of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, China (H.L., L.L., T.L., R.H., C.G.); and Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, Kansas (B.H.).
Ru HuanCollege of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, China (H.L., L.L., T.L., R.H., C.G.); and Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, Kansas (B.H.).
Bruno HagenbuchCollege of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, China (H.L., L.L., T.L., R.H., C.G.); and Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, Kansas (B.H.).ORCID 0000-0002-2938-8630
Chunshan GuiCollege of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, China (H.L., L.L., T.L., R.H., C.G.); and Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, Kansas (B.H.) guichunshan@suda.edu.cn.ORCID 0000-0001-9296-0911

Funding

Nuclear Receptors in Liver Health and DiseaseP20GM103549 · NIGMS · UNIVERSITY OF KANSAS MEDICAL CENTER · PI JAESCHKE, HARTMUT W. · 2012 to 2015
$8.1M
Molecular Characterization of Hepatic Organic Anion Transporting PolypeptidesR01GM077336 · NIGMS · UNIVERSITY OF KANSAS MEDICAL CENTER · PI HAGENBUCH, BRUNO · 2007 to 2019
$3.7M
Post-translational regulation of hepatic uptake transporters in health and diseaseR01GM149665 · NIGMS · UNIVERSITY OF KANSAS MEDICAL CENTER · PI BRUNO HAGENBUCH · 2024 to 2026
$1.2M
NIGMS NIH HHS P20 GM103549NIGMS NIH HHS R01 GM077336NIGMS NIH HHS R01 GM149665
6 · The paper itself

Abstract

Human organic anion transporting polypeptide (OATP) 1B1 and 1B3 are two highly homologous liver-specific uptake transporters. However, 2',7'-dichlorofluorescein (DCF) is preferably transported by OATP1B1. In the present study, the molecular mechanisms for the selective transport of DCF by OATP1B1 were investigated by constructing and characterizing an array of OATP1B1/1B3 chimeras and site-directed mutagenesis. Our results show that transmembrane domain (TM) 10 is crucial for the surface expression and function of OATP1B1, in which Q541 and L545 play the most important roles in DCF transport. Replacement of TM10 in OATP1B1 with its OATP1B3 counterpart led to OATP1B1's complete intracellular retention. Q541 and L545 may interact with DCF directly via hydrogen bonding and hydrophobic interactions. The decrease of DCF uptake by Q541A and L545S was due to their reduced binding affinity for DCF as compared with OATP1B1. In addition, Q541 and L545 are also crucial for the transport of estradiol-17

Indexed as

Liver-Specific Organic Anion Transporter 1Solute Carrier Organic Anion Transporter Family Member 1B3Biological TransportEstradiolEstroneFluoresceinsHEK293 CellsHumansMutagenesis, Site-DirectedOrganic Anion TransportersEstradiolestradiol-17 beta-glucuronideEstroneestrone sulfateFluoresceinsLiver-Specific Organic Anion Transporter 1Organic Anion TransportersSLCO1B1 protein, humanSLCO1B3 protein, humanSolute Carrier Organic Anion Transporter Family Member 1B3

Identifiers

PMID39209550
PMCPMC12164719

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.