Evidence map›Paper›PMID 39208730›Full record

ArticleFood chemistry2025

Comparative proteomic analysis of donor human milk treated by high-pressure processing or Holder pasteurization on undigested proteins across dynamic simulated preterm infant digestion.

Michael A Pitino, Deborah L O'Connor, Sharon Unger, Bum Jin Kim, Alain Doyen, Md Abdul Wazed, Sudarshan Kumar, Yves Pouliot, Debbie Stone, David C Dallas

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Food chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Michael A PitinoSchool of Nutrition and Public Health, College of Health, Oregon State University, Corvallis, OR, United States; Moore Family Center, College of Health, Oregon State University, Corvallis, OR, United States. Electronic address: pitinom@oregonstate.edu.
Deborah L O'ConnorDepartment of Nutritional Sciences, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada; Department of Pediatrics, Sinai Health, Toronto, Ontario, Canada; Rogers Hixon Ontario Human Milk Bank, Sinai Health, Toronto, Ontario, Canada; Translational Medicine Program, The Hospital for Sick Children, Toronto, Ontario, Canada. Electronic address: deborah.oconnor@utoronto.ca.
Sharon UngerDepartment of Pediatrics, Sinai Health, Toronto, Ontario, Canada; Rogers Hixon Ontario Human Milk Bank, Sinai Health, Toronto, Ontario, Canada; Department of Pediatrics, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada; Izaak Walton Killam Health Centre, Halifax, Nova Scotia, Canada. Electronic address: sharon.unger@iwk.nshealth.ca.
Bum Jin KimSchool of Nutrition and Public Health, College of Health, Oregon State University, Corvallis, OR, United States. Electronic address: bumjin.kim@oregonstate.edu.
Alain DoyenDépartement des Sciences des Aliments, Institut sur la nutrition et les aliments fonctionnels (INAF), Université Laval, Québec, Québec, Canada. Electronic address: alain.doyen@fsaa.ulaval.ca.
Md Abdul WazedSchool of Nutrition and Public Health, College of Health, Oregon State University, Corvallis, OR, United States. Electronic address: abdul.wazed@oregonstate.edu.
Sudarshan KumarNational Dairy Research Institute, Karnal, India.
Yves PouliotDépartement des Sciences des Aliments, Institut sur la nutrition et les aliments fonctionnels (INAF), Université Laval, Québec, Québec, Canada. Electronic address: yves.pouliot@fsaa.ulaval.ca.
Debbie StoneTranslational Medicine Program, The Hospital for Sick Children, Toronto, Ontario, Canada. Electronic address: debbie.stone@sinaihealth.ca.
David C DallasSchool of Nutrition and Public Health, College of Health, Oregon State University, Corvallis, OR, United States; Moore Family Center, College of Health, Oregon State University, Corvallis, OR, United States. Electronic address: dave.dallas@oregonstate.edu.

Funding

Effects of human milk handling practices on peptide release and bioactivity in the preterm infant intestineR01HD097367 · NICHD · OREGON STATE UNIVERSITY · PI David Charles Dallas · 2022 to 2026
$3.0M
Can high pressure processing (HPP) and ultraviolet-C irradiation (UV-C) treatment preserve donor milk bioactive protein structure and function better than holder pasteurization?R01HD106140 · NICHD · OREGON STATE UNIVERSITY · PI David Charles Dallas, Brian Scottoline · 2021 to 2026
$2.6M
Orbitrap Elite with ETDS10OD020111 · OD · OREGON STATE UNIVERSITY · PI MAIER, CLAUDIA S · 2015 to 2015
$600k
NICHD NIH HHS R01 HD097367NICHD NIH HHS R01 HD106140NIH HHS S10 OD020111
6 · The paper itself

Abstract

High-pressure processing (HPP) of donor human milk (DM) minimally impacts the concentration and bioactivity of some important bioactive proteins including lactoferrin, and bile salt-stimulated lipase (BSSL) compared to Holder pasteurization (HoP), yet the impact of HPP and subsequent digestion on the full array of proteins detectable by proteomics remains unclear. We investigated how HPP impacts undigested proteins in DM post-processing and across digestion by proteomic analysis. Each pool of milk (n = 3) remained raw, or was treated by HPP (500 MPa, 10 min) or HoP (62.5 °C, 30 min), and underwent dynamic in vitro digestion simulating the preterm infant. In the meal, major proteins were minimally changed post-processing. HPP-treated milk proteins better resisted proximal digestion (except for immunoglobulins, jejunum 180 min) and the extent of undigested proteins after gastric digestion of major proteins in HPP-treated milk was more similar to raw (e.g., BSSL, lactoferrin, macrophage-receptor-1, CD14, complement-c3/c4, xanthine dehydrogenase) than HoP.

Indexed as

DigestionInfant, PrematureMilk, HumanMilk ProteinsPasteurizationProteomicsFemaleFood HandlingHumansInfantInfant, NewbornLactoferrinModels, BiologicalPressureLactoferrinMilk ProteinsBioactive proteinsBreastmilkDynamic in vitro digestionHigh hydrostatic pressureLow-temperature long-time pasteurizationNon-thermal processing

Identifiers

PMID39208730
PMCPMC11413619

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.