ArticleScience (New York, N.Y.)2024
Phage-triggered reverse transcription assembles a toxic repetitive gene from a noncoding RNA.
Article in Science (New York, N.Y.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Evolutionary dynamics and research hotspots of phage applications againstFrontiers in microbiology · 2026Pooled it
- Defense-associated reverse transcriptases define an emerging class of noncanonical DNA-writing systems.Synthetic and systems biotechnology · 2026Article
- Article
- Recent Advances in the Molecular Mechanisms of Bacterial Anti-Phage Defence Systems.Microbial biotechnology · 2026Article
- Coordinated RNA- and protein-templated synthesis of double-stranded DNA by a dual reverse transcriptase immune system.bioRxiv : the preprint server for biology · 2026Article
- Protein-templated synthesis of dinucleotide repeat DNA by an antiphage reverse transcriptase.Science (New York, N.Y.) · 2026Article
- High-throughput functional profiling and evolutionary covariation analysis of entire riboswitch sequences.Nucleic acids research · 2026Article
- Semirandom DNA adducts regulate a filamentous defense-associated reverse transcriptase.Nature structural & molecular biology · 2026Article
- Construction of synthetic protein-binding non-genetic DNA systems in living cells.Nature chemistry · 2026Article
- DefensePredictor: A machine learning model to discover prokaryotic immune systems.Science (New York, N.Y.) · 2026Article
- Article
- Review
- Bacterial defense systems: Mechanisms, homology to eukaryotic immune systems, and applications.Zoological research · 2026Review
- Protein-directed nucleotide selection in the DRT3 defense system: mechanism, biological context, and constraints on programmability.Frontiers in microbiology · 2026Review
- Anti-phage defense mechanism involving phage-encoded DNA binding protein and bacterial reverse transcriptase DRT4.Nature communications · 2025Article
- High-throughput functional profiling and evolutionary covariation analysis of entire riboswitch sequences.bioRxiv : the preprint server for biology · 2025Article
- Phage nuclease-mediated defense activation of the bacterial Retron-Eco7 toxin-antitoxin system.Nucleic acids research · 2025Article
- Bacterial restriction-modification systems: mechanisms of defense against phage infection.Biophysics reports · 2025Article
- Molecular mechanism of the type 2 defense-associated reverse transcriptase.Nucleic acids research · 2025Article
- Antiviral reverse transcriptases reveal the evolutionary origin of telomerase.bioRxiv : the preprint server for biology · 2025Article
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5 authors.
Funding
Abstract
Reverse transcription has frequently been co-opted for cellular functions and in prokaryotes is associated with protection against viral infection, but the underlying mechanisms of defense are generally unknown. Here, we show that in the DRT2 defense system, the reverse transcriptase binds a neighboring pseudoknotted noncoding RNA. Upon bacteriophage infection, a template region of this RNA is reverse transcribed into an array of tandem repeats that reconstitute a promoter and open reading frame, allowing expression of a toxic repetitive protein and an abortive infection response. Biochemical reconstitution of this activity and cryo-electron microscopy provide a molecular basis for repeat synthesis. Gene synthesis from a noncoding RNA is a previously unknown mode of genetic regulation in prokaryotes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.