Evidence map›Paper›PMID 39207937›Full record

ArticlePLoS pathogens2024

Marine sponge microbe provides insights into evolution and virulence of the tubercle bacillus.

Sacha J Pidot, Stephan Klatt, Louis S Ates, Wafa Frigui, Fadel Sayes, Laleh Majlessi, Hiroshi Izumi, Ian R Monk, Jessica L Porter, Vicki Bennett-Wood and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS pathogens, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Detection ofApplied and environmental microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Sacha J PidotDepartment of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia.ORCID 0000-0003-1202-6614
Stephan KlattDepartment of Molecular Biology and Biochemistry, Bio21 Institute, University of Melbourne, Parkville, Australia.
Louis S AtesInstitut Pasteur, Université Paris Cité, Unit for Integrated Mycobacterial Pathogenomics, Paris, France.
Wafa FriguiInstitut Pasteur, Université Paris Cité, Unit for Integrated Mycobacterial Pathogenomics, Paris, France.
Fadel SayesInstitut Pasteur, Université Paris Cité, Unit for Integrated Mycobacterial Pathogenomics, Paris, France.
Laleh MajlessiInstitut Pasteur, Université Paris Cité, Unit for Integrated Mycobacterial Pathogenomics, Paris, France.
Hiroshi IzumiSchool of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Australia.
Ian R MonkDepartment of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia.
Jessica L PorterDepartment of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia.
Vicki Bennett-WoodDepartment of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia.
Torsten SeemannDepartment of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia.
Ashley OtterUK Health Security Agency, Porton Down, Salisbury, United Kingdom.
George TaiaroaDepartment of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
Gregory M CookDepartment of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
Nicholas WestSchool of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Australia.
Nicholas J TobiasDepartment of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia.
John A FuerstSchool of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Australia.
Michael D StutzWalter and Eliza Hall Institute of Medical Research, Parkville, Australia.
Marc PellegriniWalter and Eliza Hall Institute of Medical Research, Parkville, Australia.
Malcolm McConvilleDepartment of Molecular Biology and Biochemistry, Bio21 Institute, University of Melbourne, Parkville, Australia.
Roland BroschInstitut Pasteur, Université Paris Cité, Unit for Integrated Mycobacterial Pathogenomics, Paris, France.
Timothy P StinearDepartment of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia.

Funding

Australian Research Council (ARC) LP0882233National Health and Medical Research Council (NHMRC) ANR-10-LABX-62-IBEIDNational Health and Medical Research Council (NHMRC) GNT1105522National Health and Medical Research Council (NHMRC) GNT2021638
6 · The paper itself

Abstract

Reconstructing the evolutionary origins of Mycobacterium tuberculosis, the causative agent of human tuberculosis, has helped identify bacterial factors that have led to the tubercle bacillus becoming such a formidable human pathogen. Here we report the discovery and detailed characterization of an exceedingly slow growing mycobacterium that is closely related to M. tuberculosis for which we have proposed the species name Mycobacterium spongiae sp. nov., (strain ID: FSD4b-SM). The bacterium was isolated from a marine sponge, taken from the waters of the Great Barrier Reef in Queensland, Australia. Comparative genomics revealed that, after the opportunistic human pathogen Mycobacterium decipiens, M. spongiae is the most closely related species to the M. tuberculosis complex reported to date, with 80% shared average nucleotide identity and extensive conservation of key M. tuberculosis virulence factors, including intact ESX secretion systems and associated effectors. Proteomic and lipidomic analyses showed that these conserved systems are functional in FSD4b-SM, but that it also produces cell wall lipids not previously reported in mycobacteria. We investigated the virulence potential of FSD4b-SM in mice and found that, while the bacteria persist in lungs for 56 days after intranasal infection, no overt pathology was detected. The similarities with M. tuberculosis, together with its lack of virulence, motivated us to investigate the potential of FSD4b-SM as a vaccine strain and as a genetic donor of the ESX-1 genetic locus to improve BCG immunogenicity. However, neither of these approaches resulted in superior protection against M. tuberculosis challenge compared to BCG vaccination alone. The discovery of M. spongiae adds to our understanding of the emergence of the M. tuberculosis complex and it will be another useful resource to refine our understanding of the factors that shaped the evolution and pathogenesis of M. tuberculosis.

Indexed as

Aquatic OrganismsBiological EvolutionMycobacterium tuberculosisPoriferaAnimalsBacterial ProteinsDNA MethylationGenomicsLipidsMice, Inbred C57BLTuberculosis VaccinesVirulenceBacterial ProteinsLipidsTuberculosis Vaccines

Identifiers

PMID39207937
PMCPMC11361433

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.