Evidence map›Paper›PMID 39207902›Full record

ArticleCell reports2024

Confinement controls the directional cell responses to fluid forces.

Farshad Amiri, Ayuba A Akinpelu, William C Keith, Farnaz Hemmati, Ravi S Vaghasiya, Dylan Bowen, Razan S Waliagha, Chuanyu Wang, Pengyu Chen, Amit K Mitra and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Farshad AmiriDepartment of Chemical Engineering, Auburn University, Auburn, AL 36849, USA.
Ayuba A AkinpeluDepartment of Chemical Engineering, Auburn University, Auburn, AL 36849, USA.
William C KeithDepartment of Chemical Engineering, Auburn University, Auburn, AL 36849, USA.
Farnaz HemmatiDepartment of Chemical Engineering, Auburn University, Auburn, AL 36849, USA.
Ravi S VaghasiyaDepartment of Chemical Engineering, Auburn University, Auburn, AL 36849, USA.
Dylan BowenDepartment of Chemical Engineering, Auburn University, Auburn, AL 36849, USA.
Razan S WaliaghaDepartment of Drug Discovery and Development, Harrison College of Pharmacy, Auburn University, Auburn, AL 36849, USA.
Chuanyu WangMaterials Research and Education Center, Auburn University, Auburn, AL 36849, USA.
Pengyu ChenMaterials Research and Education Center, Auburn University, Auburn, AL 36849, USA.
Amit K MitraDepartment of Drug Discovery and Development, Harrison College of Pharmacy, Auburn University, Auburn, AL 36849, USA; Center for Pharmacogenomics and Single-Cell Omics (AUPharmGx), Harrison College of Pharmacy, Auburn University, Auburn, AL 36849, USA; UAB O'Neal Comprehensive Cancer, University of Alabama at Birmingham School of Medicine, Birmingham, AL 35233, USA.
Yizeng LiDepartment of Biomedical Engineering, Binghamton University, SUNY, Binghamton, NY 13902, USA.
Panagiotis MistriotisDepartment of Chemical Engineering, Auburn University, Auburn, AL 36849, USA. Electronic address: pmistriotis@auburn.edu.

Funding

Purchase of a modularized confocal microscopeR35GM133795 · NIGMS · AUBURN UNIVERSITY AT AUBURN · PI Pengyu Chen · 2019 to 2026
$3.3M
Equipment Supplement to R35GM133794R35GM133794 · NIGMS · UNIVERSITY OF KANSAS LAWRENCE · PI Mei He · 2019 to 2026
$2.9M
Cell mechanoresponses in physiologically relevant microenvironmentsR35GM147101 · NIGMS · AUBURN UNIVERSITY AT AUBURN · PI Panagiotis Mistriotis · 2022 to 2026
$2.1M
NIGMS NIH HHS R35 GM133794NIGMS NIH HHS R35 GM133795NIGMS NIH HHS R35 GM147101
6 · The paper itself

Abstract

Our understanding of how fluid forces influence cell migration in confining environments remains limited. By integrating microfluidics with live-cell imaging, we demonstrate that cells in tightly-but not moderately-confined spaces reverse direction and move upstream upon exposure to fluid forces. This fluid force-induced directional change occurs less frequently when cells display diminished mechanosensitivity, experience elevated hydraulic resistance, or sense a chemical gradient. Cell reversal requires actin polymerization to the new cell front, as shown mathematically and experimentally. Actin polymerization is necessary for the fluid force-induced activation of NHE1, which cooperates with calcium to induce upstream migration. Calcium levels increase downstream, mirroring the subcellular distribution of myosin IIA, whose activation enhances upstream migration. Reduced lamin A/C levels promote downstream migration of metastatic tumor cells by preventing cell polarity establishment and intracellular calcium rise. This mechanism could allow cancer cells to evade high-pressure environments, such as the primary tumor.

Indexed as

ActinsCalciumCell MovementCell Line, TumorCell PolarityHumansLamin Type AMechanotransduction, CellularMicrofluidicsSodium-Hydrogen Exchanger 1ActinsCalciumLamin Type ASodium-Hydrogen Exchanger 1actin polymerizationactomyosin contractilitycancer metastasischemotaxisconfined migrationCP: Cell biologyhydraulic resistancemechanosensitive ion channelsNHE1nuclear stiffnessupstream migration

Identifiers

PMID39207902
PMCPMC11495937

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.