Evidence map›Paper›PMID 39207519›Full record

ArticleHistochemistry and cell biology2024

Identification and culture of functional salivary gland ductal epithelial cells.

Han-Shu Zhang, You-Wei Zhao, Xin-Yi Tao, Xin Cong, Li-Ling Wu, Guang-Yan Yu, Yan Zhang

Abstract read
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In one paragraph

Article in Histochemistry and cell biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Han-Shu ZhangDepartment of Oral and Maxillofacial Surgery, Biomaterials and Digital Medical Devices, Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health, NMPA Key Laboratory for Dental Materials, Peking University School and Hospital of Stomatology, National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Research Center of Oral, 22 Zhong Guan Cun South Street, Haidian District, Beijing, 100081, China.
You-Wei ZhaoDepartment of Oral and Maxillofacial Surgery, Biomaterials and Digital Medical Devices, Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health, NMPA Key Laboratory for Dental Materials, Peking University School and Hospital of Stomatology, National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Research Center of Oral, 22 Zhong Guan Cun South Street, Haidian District, Beijing, 100081, China.
Xin-Yi TaoDepartment of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Haidian District, Beijing, 100191, China.
Xin CongDepartment of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Haidian District, Beijing, 100191, China.
Li-Ling WuDepartment of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Haidian District, Beijing, 100191, China.
Guang-Yan YuDepartment of Oral and Maxillofacial Surgery, Biomaterials and Digital Medical Devices, Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health, NMPA Key Laboratory for Dental Materials, Peking University School and Hospital of Stomatology, National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Research Center of Oral, 22 Zhong Guan Cun South Street, Haidian District, Beijing, 100081, China. gyyu@263.net.
Yan ZhangDepartment of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 38 Xueyuan Road, Haidian District, Beijing, 100191, China. zhangy18@bjmu.edu.cn.

Funding

National Natural Science Foundation of China 82270993Natural Science Foundation of Beijing Municipality 7212062
6 · The paper itself

Abstract

Sialadenitis is a prevalent salivary gland disease resulting in decreased salivary flow rate. To date, little is known about the exact changes and mechanism of ductal cells in sialadenitis. This study aims to establish an efficient method to identify and isolate ductal cells, thereby facilitating further research on this specific cell type. Immunofluorescence for cytokeratin 13 and cytokeratin 19 was conducted in salivary glands to confirm their specificity as ductal cell markers. The dissected ducts were assessed through PCR and Western blot of cytokeratin 19 and digested by dispase and collagenase. The functionality of the isolated ductal cells was determined by measuring intracellular calcium. Cytokeratin 19 and cytokeratin 13 were expressed in all segments of human ducts. Cytokeratin 19 was limited to ducts excluding granular convoluted tubules in rat and mouse. The purities of the obtained ductal cells were approximately 98% in humans and 93% in rats. Furthermore, intracellular free calcium increased with time and concentration of carbachol treatment. Cytokeratin 19 serves as a dependable marker for identifying ductal cells in salivary glands, except for granular convoluted tubules. Moreover, we have successfully developed an efficient method for isolating ductal cells from salivary glands.

Indexed as

Epithelial CellsSalivary GlandsAdultAnimalsCalciumCells, CulturedFemaleHumansKeratin-19MaleMiceMiddle AgedRatsRats, Sprague-DawleySalivary DuctsCalciumKeratin-19CalciumCytokeratinDuctSalivary gland

Identifiers

PMID39207519

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.