Evidence map›Paper›PMID 39206452›Full record

ArticleBiology methods & protocols2024

Generating CRISPR-edited clonal lines of cultured

John M Ryniawec, Anastasia Amoiroglou, Gregory C Rogers

Abstract read
In one paragraph

Article in Biology methods & protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

John M RyniawecDepartment of Cellular and Molecular Medicine, University of Arizona Cancer Center, University of Arizona, Tucson, AZ 85724, United States.ORCID https://orcid.org/0000-0003-3073-7831
Anastasia AmoiroglouDepartment of Cellular and Molecular Medicine, University of Arizona Cancer Center, University of Arizona, Tucson, AZ 85724, United States.
Gregory C RogersDepartment of Cellular and Molecular Medicine, University of Arizona Cancer Center, University of Arizona, Tucson, AZ 85724, United States.ORCID https://orcid.org/0000-0003-1870-1713

Funding

VITAMIN AP30CA023074 · NCI · UNIVERSITY OF ARIZONA · PI Dan Theodorescu · 1985 to 2026
$110.2M
Characterizing the molecular mechanisms of centriole duplication, growth and maturationR35GM136265 · NIGMS · UNIVERSITY OF ARIZONA · PI Gregory Charles Rogers · 2020 to 2026
$3.9M
NCI NIH HHS P30 CA023074NIGMS NIH HHS R35 GM136265
6 · The paper itself

Abstract

CRISPR/Cas9 genome editing is a pervasive research tool due to its relative ease of use. However, some systems are not amenable to generating edited clones due to genomic complexity and/or difficulty in establishing clonal lines. For example,

Indexed as

Cas9centriolecentrosomeCRISPR genome editingDrosophilamicrotubulePolo-like kinase 4/Plk4Schneider S2 cells

Identifiers

PMID39206452
PMCPMC11357795

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.