Evidence map›Paper›PMID 39205660›Full record

ArticleAmerican journal of physiology. Renal physiology2024

Optimizing renal transporter immunodetection: consequences of freeze-thaw during sample preparation.

Hannah L Hartman-Houstman, Donna L Ralph, Jonathan W Nelson, Lawrence G Palmer, Jessica E Faulkner, Jennifer C Sullivan, Desmond M Moronge, Alicia A McDonough

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Renal physiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hannah L Hartman-HoustmanDepartment of Physiology and Neuroscience, Keck School of Medicine, University of Southern California, Los Angeles, California, United States.
Donna L RalphDepartment of Physiology and Neuroscience, Keck School of Medicine, University of Southern California, Los Angeles, California, United States.
Jonathan W NelsonDivision of Nephrology and Hypertension, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California, United States.
Lawrence G PalmerDepartment of Physiology and Biophysics, Weill Cornell Medicine, New York, New York, United States.ORCID 0000-0001-7702-4703
Jessica E FaulknerDepartment of Physiology, Medical College of Georgia at Augusta University, Augusta, Georgia, United States.ORCID 0000-0003-3362-412X
Jennifer C SullivanDepartment of Physiology, Medical College of Georgia at Augusta University, Augusta, Georgia, United States.ORCID 0000-0003-0541-0247
Desmond M MorongeDepartment of Physiology, Medical College of Georgia at Augusta University, Augusta, Georgia, United States.ORCID 0000-0001-8134-0645
Alicia A McDonoughDepartment of Physiology and Neuroscience, Keck School of Medicine, University of Southern California, Los Angeles, California, United States.ORCID 0000-0002-0459-469X

Funding

Sodium transporter regulation during hypertensionR01DK083785 · NIDDK · UNIVERSITY OF SOUTHERN CALIFORNIA · PI AURELIE EDWARDS, Alicia A. McDonough · 2011 to 2026
$5.8M
Mechanisms of subclinical renal injury in females following AKI: implications for adverse pregnancy outcomesR01DK134695 · NIDDK · AUGUSTA UNIVERSITY · PI Jennifer C Sullivan · 2023 to 2026
$1.8M
Sodium-chloride co-transporter regulation in the kidneyR56DK083785 · NIDDK · UNIVERSITY OF SOUTHERN CALIFORNIA · PI MCDONOUGH, ALICIA A., YU, ALAN S · 2010 to 2010
$173k
HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) DK083785HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) DK134695NIDDK NIH HHS R01 DK083785NIDDK NIH HHS R01 DK134695NIDDK NIH HHS R56 DK083785
6 · The paper itself

Abstract

Renal transporters (cotransporters, channels, and claudins) mediate homeostasis of fluids and electrolytes and are targets of hormonal and therapeutic regulators. Assessing renal transporter abundance with antibody probes by immunoblotting is an essential tool for mechanistic studies. Although journals require authors to demonstrate antibody specificity, there are no consensus guidelines for kidney sample preparation leading to lab-to-lab variability in immunoblot results. In this study, we determined the impact of sample preparation, specifically freeze-thawed (Frozen) versus freshly processed (Fresh) kidneys (female and male rats and mice) on immunoblot signal detection of 15 renal transporters and the impact of protease inhibitors during homogenization. In female Sprague-Dawley rat kidneys homogenized with aprotinin, Na

Indexed as

KidneyMice, Inbred C57BLRats, Sprague-DawleyAnimalsFemaleFreezingMaleMembrane Transport ProteinsMiceProtease InhibitorsRatsMembrane Transport ProteinsProtease Inhibitorsfreeze-thawimmunoblottingrenal transportersrigor and reproducibility

Identifiers

PMID39205660
PMCPMC11483075

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.