Evidence map›Paper›PMID 39203517›Full record

ArticleMicroorganisms2024

Molecular Investigation of Small Ruminant Abortions Using a 10-Plex HRM-qPCR Technique: A Novel Approach in Routine Diagnostics.

Ioannis Gouvias, Marios Lysitsas, Apostolos Batsidis, Sonia Malefaki, Dimitra Bitchava, Anna Tsara, Emilija Nickovic, Ilias Bouzalas, Eleni Malissiova, Raphaël Guatteo and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microorganisms, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Microorganisms · 2026
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ioannis GouviasFaculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece.
Marios LysitsasFaculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece.ORCID 0009-0007-0648-6004
Apostolos BatsidisDepartment of Mathematics, University of Ioannina, 45110 Ioannina, Greece.ORCID 0000-0002-4491-4387
Sonia MalefakiDepartment of Mechanical Engineering and Aeronautics, University of Patras, 26500 Rion-Patras, Greece.ORCID 0000-0002-0756-3557
Dimitra BitchavaVet in Progress Plus, Veterinary Laboratories, 15343 Athens, Greece.
Anna TsaraVet in Progress Plus, Veterinary Laboratories, 15343 Athens, Greece.
Emilija NickovicFaculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece.
Ilias BouzalasVeterinary Research Institute, Hellenic Agricultural Organization DIMITRA (ELGO-DIMITRA), Campus Thermi, 57001 Thessaloniki, Greece.ORCID 0000-0002-3018-1426
Eleni MalissiovaFood of Animal Origin Laboratory, Animal Science Department, University of Thessaly, 41500 Larissa, Greece.ORCID 0000-0001-8956-2269
Raphaël GuatteoBIOEPAR, INRAE Oniris, 44300 Nantes, France.ORCID 0000-0002-6658-522X
George ValiakosFaculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece.ORCID 0000-0002-3869-5026

Funding

CEVA Hellas 7652 (University of Thessaly)
6 · The paper itself

Abstract

The objective of this study was to apply and preliminarily evaluate a High-Resolution Melting (HRM) analysis technique coupled with qPCR, that allows the simultaneous detection of 10 different ruminant abortogenic pathogens, for investigating abortions in sheep and goats throughout Greece. A total of 264 ovine and caprine vaginal swabs were obtained the week following the abortion from aborted females and analyzed using a commercially available kit (ID Gene™ Ruminant Abortion Multiplex HRM, Innovative Diagnostics). Results indicated a high prevalence of

Indexed as

abortogenic pathogensAnaplasma phagocytophilumBrucella spp.Campylobacter fetusChlamydophila spp.Coxiella burnetiiGreeceHRMNeospora caninumqPCRSalmonella spp.small ruminantsToxoplasma gondii

Identifiers

PMID39203517
PMCPMC11356958

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.