Evidence map›Paper›PMID 39202288›Full record

ArticleDiagnostics (Basel, Switzerland)2024

Average Nucleotide Identity and Digital DNA-DNA Hybridization Analysis Following PromethION Nanopore-Based Whole Genome Sequencing Allows for Accurate Prokaryotic Typing.

Nick Versmessen, Marieke Mispelaere, Marjolein Vandekerckhove, Cedric Hermans, Jerina Boelens, Katleen Vranckx, Filip Van Nieuwerburgh, Mario Vaneechoutte, Paco Hulpiau, Piet Cools

Abstract read
In one paragraph

Article in Diagnostics (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Pangenomic and genomic plasticity analyses of the genus Rickettsia.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Genomic characterization ofMicrobial genomics · 2025
    Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Nick VersmessenLaboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.ORCID 0000-0001-9829-8236
Marieke MispelaereDepartment of Bio-Medical Sciences, HOWEST University of Applied Sciences, 8000 Bruges, Belgium.ORCID 0009-0005-3004-9424
Marjolein VandekerckhoveDepartment of Bio-Medical Sciences, HOWEST University of Applied Sciences, 8000 Bruges, Belgium.
Cedric HermansDepartment of Bio-Medical Sciences, HOWEST University of Applied Sciences, 8000 Bruges, Belgium.ORCID 0000-0002-9310-1876
Jerina BoelensDepartment of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.
Katleen VranckxBioMérieux, 1030 Brussels, Belgium.ORCID 0000-0002-1980-9809
Filip Van NieuwerburghNXTGNT, Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Ghent University, 9000 Ghent, Belgium.ORCID 0000-0001-8815-5485
Mario VaneechoutteLaboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.ORCID 0000-0002-4586-203X
Paco HulpiauDepartment of Bio-Medical Sciences, HOWEST University of Applied Sciences, 8000 Bruges, Belgium.ORCID 0000-0002-6692-2150
Piet CoolsLaboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium.ORCID 0000-0003-2980-5307

Funding

Research Foundation - Flanders 1S97421NSpecial Research Fund of Ghent University BOF.COV.2020.0009.01
6 · The paper itself

Abstract

Whole-genome sequencing (WGS) is revolutionizing clinical bacteriology. However, bacterial typing remains investigated by reference techniques with inherent limitations. This stresses the need for alternative methods providing robust and accurate sequence type (ST) classification. This study optimized and evaluated a GridION nanopore sequencing protocol, adapted for the PromethION platform. Forty-eight

Indexed as

antibiotic resistanceaverage nucleotide identityclinical bacteriologydigital DNA-DNA hybridizationEscherichia coliminimum inhibitory concentrationmulti-locus sequence typingnanopore sequencingspecies delineationwhole genome sequencing

Identifiers

PMID39202288
PMCPMC11353866

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.