Evidence map›Paper›PMID 39201641›Full record

ArticleInternational journal of molecular sciences2024

Potency Evaluations of Recombinant Botulinum Neurotoxin A1 Mutants Designed to Reduce Toxicity.

Polrit Viravathana, William H Tepp, Marite Bradshaw, Amanda Przedpelski, Joseph T Barbieri, Sabine Pellett

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Neuroscience in prostate cancer.Prostate cancer and prostatic diseases · 2026
    Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Polrit ViravathanaDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
William H TeppDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Marite BradshawDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Amanda PrzedpelskiDepartment of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Joseph T BarbieriDepartment of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Sabine PellettDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.ORCID 0000-0001-8186-8810

Funding

Characteristics of Botulinum Neurotoxins the Determine PotencyR01AI139306 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI Joseph T Barbieri, Sabine Pellett · 2019 to 2026
$5.5M
NIAID NIH HHS R01 AI139306NIAID NIH HHS R01AI139306University of Wisconsin-Madison Food Research Institute n/a
6 · The paper itself

Abstract

Recombinant mutant holotoxin BoNTs (rBoNTs) are being evaluated as possible vaccines against botulism. Previously, several rBoNTs containing 2-3 amino acid mutations in the light chain (LC) showed significant decreases in toxicity (2.5-million-fold-12.5-million-fold) versus wild-type BoNT/A1, leading to their current exclusion from the Federal Select Agent list. In this study, we added four additional mutations in the receptor-binding domain, translocation domain, and enzymatic cleft to further decrease toxicity, creating 7M rBoNT/A1. Due to poor expression in

Indexed as

Botulinum Toxins, Type AMutationRecombinant ProteinsAmino Acid SubstitutionAnimalsClostridium botulinumEscherichia coliMiceBotulinum Toxins, Type ARecombinant ProteinsatoxicBoNTbotulinum neurotoxinendogenous expressionexpression systemrecombinant

Identifiers

PMID39201641
PMCPMC11355004

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.