Evidence map›Paper›PMID 39201420›Full record

ArticleInternational journal of molecular sciences2024

Recombinant Subunit Vaccine Candidate against the Bovine Viral Diarrhea Virus.

Verónica Avello, Santiago Salazar, Eddy E González, Paula Campos, Viana Manríque, Christian Mathieu, Florence Hugues, Ignacio Cabezas, Paula Gädicke, Natalie C Parra and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Verónica AvelloBiotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción P.O. Box 160C, Chile.ORCID 0000-0002-5292-8295
Santiago SalazarBiotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción P.O. Box 160C, Chile.ORCID 0000-0003-2614-1218
Eddy E GonzálezDepartment of Medicine, Division of Gastroenterology, Miller School of Medicine, University of Miami, Miami, FL 33146, USA.ORCID 0000-0003-2043-7552
Paula CamposBiotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción P.O. Box 160C, Chile.
Viana ManríqueBiotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción P.O. Box 160C, Chile.ORCID 0000-0003-0140-1861
Christian MathieuVirology Section of the SAG's Sub-Department Network of Animal Health Laboratories, Lo Aguirre, Santiago de Chile 9020000, Chile.
Florence HuguesPathology and Preventive Medicine Department, School of Veterinary Sciences, Universidad de Concepción, Vicente Méndez 595, Chillán P.O. Box 537, Chile.ORCID 0000-0002-2283-6269
Ignacio CabezasPathology and Preventive Medicine Department, School of Veterinary Sciences, Universidad de Concepción, Vicente Méndez 595, Chillán P.O. Box 537, Chile.ORCID 0000-0001-8118-2579
Paula GädickePathology and Preventive Medicine Department, School of Veterinary Sciences, Universidad de Concepción, Vicente Méndez 595, Chillán P.O. Box 537, Chile.ORCID 0000-0001-6915-1446
Natalie C ParraBiotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción P.O. Box 160C, Chile.
Jannel AcostaBiotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción P.O. Box 160C, Chile.
Oliberto SánchezPharmacology Department, School of Biological Sciences, Universidad de Concepción, Victor Lamas 1290, Concepción P.O. Box 160C, Chile.
Alaín GonzálezFaculty of Basic Sciences, University of Medellin, Cra. 87 No 30-65, Medellin 050026, Colombia.
Raquel MontesinoBiotechnology and Biopharmaceuticals Laboratory, Pathophysiology Department, School of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción P.O. Box 160C, Chile.

Funding

Fondo Nacional de Desarrollo Científico y Tecnológico ID21I10020
6 · The paper itself

Abstract

Multivalent live-attenuated or inactivated vaccines are often used to control the bovine viral diarrhea disease (BVD). Still, they retain inherent disadvantages and do not provide the expected protection. This study developed a new vaccine prototype, including the external segment of the E2 viral protein from five different subgenotypes selected after a massive screening. The E2 proteins of every subgenotype (1aE2, 1bE2, 1cE2, 1dE2, and 1eE2) were produced in mammalian cells and purified by IMAC. An equimolar mixture of E2 proteins formulated in an oil-in-water adjuvant made up the vaccine candidate, inducing a high humoral response at 50, 100, and 150 µg doses in sheep. A similar immune response was observed in bovines at 50 µg. The cellular response showed a significant increase in the transcript levels of relevant Th1 cytokines, while those corresponding to the Th2 cytokine IL-4 and the negative control were similar. High levels of neutralizing antibodies against the subgenotype BVDV1a demonstrated the effectiveness of our vaccine candidate, similar to that observed in the sera of animals vaccinated with the commercial vaccine. These results suggest that our vaccine prototype could become an effective recombinant vaccine against the BVD.

Indexed as

Antibodies, ViralBovine Virus Diarrhea-Mucosal DiseaseVaccines, SubunitVaccines, SyntheticViral VaccinesAnimalsAntibodies, NeutralizingCattleCytokinesDiarrhea Virus 1, Bovine ViralDiarrhea Viruses, Bovine ViralSheepViral Envelope ProteinsAntibodies, NeutralizingAntibodies, ViralCytokinesVaccines, SubunitVaccines, SyntheticViral Envelope ProteinsViral Vaccinesbovine viral diarrhea virusBVDV subgenotypesE2 glycoproteinsubunit vaccine

Identifiers

PMID39201420
PMCPMC11354329

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.