Evidence map›Paper›PMID 39201364›Full record

ArticleInternational journal of molecular sciences2024

A Combined Transcriptomic and Proteomic Analysis of Monkeypox Virus A23 Protein on HEK293T Cells.

Yihao Wang, Yihan Li, Mingzhi Li, Keyi Wang, Jiaqi Xiong, Ting Wang, Yu Wang, Yunli Guo, Lingbao Kong, Meifeng Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yihao WangInstitute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330000, China.
Yihan LiInstitute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330000, China.
Mingzhi LiInstitute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330000, China.
Keyi WangInstitute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330000, China.
Jiaqi XiongInstitute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330000, China.
Ting WangInstitute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330000, China.
Yu WangInstitute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330000, China.ORCID 0009-0008-9251-344X
Yunli GuoInstitute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330000, China.
Lingbao KongInstitute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330000, China.
Meifeng LiInstitute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330000, China.

Funding

Jiangxi Agricultural University No. GJJ2200442 and 20224BAB215036National Natural Science Foundation of China 32360908
6 · The paper itself

Abstract

Monkeypox virus (MPXV) is a cross-kingdom pathogen infecting both humans and wildlife, which poses a significant health risk to the public. Although MPXV attracts broad attention, there is a lack of adequate studies to elucidate pathogenic mechanisms associated with viral infections. In this study, a high-throughput RNA sequencing (RNA-seq) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach was used to explore the transcriptional and metabolic responses of MPXV A23 protein to HEK293T cells. The protein-protein interactions and signaling pathways were conducted by GO and KEGG analyses. The localization of A23 protein in HEK293T cells was detected by immunofluorescence. A total of 648 differentially expressed genes (DEGs) were identified in cells by RNA-Seq, including 314 upregulated genes and 334 downregulated genes. Additionally, liquid chromatography-tandem mass spectrometry (LC-MS/MS) detected 115 cellular proteins that interact with the A23 proteins. Transcriptomic sequencing analysis revealed that transfection of MPXV A23 protein modulated genes primarily associated with cellular apoptosis and DNA damage repair. Proteomic analysis indicated that this protein primarily interacted with host ribosomal proteins and histones. Following the identification of the nuclear localization sequence RKKR within the A23 protein, a truncated mutant A23

Indexed as

Monkeypox virusProteomicsTranscriptomeViral ProteinsChromatography, LiquidGene Expression ProfilingHEK293 CellsHumansProteomeTandem Mass SpectrometryProteomeViral ProteinsA23Rinnate immune signaling pathwaympox virusproteomictranscriptome

Identifiers

PMID39201364
PMCPMC11354578

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.