Evidence map›Paper›PMID 39201242›Full record

ArticleInternational journal of molecular sciences2024

A Comprehensive ddPCR Strategy for Sensitive and Reliable Monitoring of CAR-T Cell Kinetics in Clinical Applications.

Gertrud Wiedemann, Ulrike Bacher, Raphael Joncourt, Françoise Solly, Corinne C Widmer, Sacha Zeerleder, Urban Novak, Thomas Pabst, Naomi A Porret

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Trial
  2. Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Gertrud WiedemannDepartment of Hematology and Central Hematological Laboratory, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, Switzerland.ORCID 0000-0002-2259-1011
Ulrike BacherDepartment of Hematology and Central Hematological Laboratory, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, Switzerland.ORCID 0000-0001-8771-947X
Raphael JoncourtDepartment of Hematology and Central Hematological Laboratory, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, Switzerland.
Françoise SollyService and Central Laboratory of Hematology, Lausanne University Hospital (CHUV), 1011 Lausanne, Switzerland.
Corinne C WidmerDepartment of Medical Oncology and Hematology, University Hospital Zurich, 8091 Zurich, Switzerland.
Sacha ZeerlederDepartment for BioMedical Research, University of Bern, 3008 Bern, Switzerland.
Urban NovakDepartment of Medical Oncology, Inselspital, University Hospital of Bern, 3010 Bern, Switzerland.
Thomas PabstDepartment of Medical Oncology, Inselspital, University Hospital of Bern, 3010 Bern, Switzerland.ORCID 0000-0002-6055-5257
Naomi A PorretDepartment of Hematology and Central Hematological Laboratory, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, Switzerland.ORCID 0000-0002-2515-1935

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we present the design, implementation, and successful use of digital droplet PCR (ddPCR) for the monitoring of chimeric antigen receptor T-cell (CAR-T) expansion in patients with B-cell malignancies treated with different CAR-T products at our clinical center. Initially, we designed a specific and highly sensitive ddPCR assay targeting the junction between the 4-1BB and CD3ζ domains of tisa-cel, normalized with

Indexed as

Immunotherapy, AdoptiveReceptors, Chimeric AntigenT-LymphocytesHumansKineticsPolymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificityReceptors, Chimeric Antigenacute lymphatic leukemia (ALL)B-cell lymphomaCAR-T cell therapiesdroplet digital PCR (ddPCR)molecular monitoring

Identifiers

PMID39201242
PMCPMC11354041

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.