Evidence map›Paper›PMID 39199837›Full record

ArticleAnimals : an open access journal from MDPI2024

Gene Expression Analysis before and after the Pelvic Flexure in the Epithelium of the Equine Hindgut.

Cameron D Moss, Amber L Wilson, Kailee J Reed, Kaysie J Jennings, Isabelle G Z Kunz, Gabriele A Landolt, Jessica Metcalf, Terry E Engle, Stephen J Coleman

Abstract read
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In one paragraph

Article in Animals : an open access journal from MDPI, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Cameron D MossDepartment of Animal Sciences, Colorado State University, Fort Collins, CO 8023, USA.
Amber L WilsonDepartment of Animal Sciences, Colorado State University, Fort Collins, CO 8023, USA.
Kailee J ReedDepartment of Animal Sciences, Colorado State University, Fort Collins, CO 8023, USA.
Kaysie J JenningsDepartment of Animal Sciences, Colorado State University, Fort Collins, CO 8023, USA.
Isabelle G Z KunzDepartment of Animal Sciences, Colorado State University, Fort Collins, CO 8023, USA.
Gabriele A LandoltDepartment of Clinical Sciences, Colorado State University, Fort Collins, CO 8023, USA.
Jessica MetcalfDepartment of Animal Sciences, Colorado State University, Fort Collins, CO 8023, USA.
Terry E EngleDepartment of Animal Sciences, Colorado State University, Fort Collins, CO 8023, USA.
Stephen J ColemanDepartment of Animal Sciences, Colorado State University, Fort Collins, CO 8023, USA.ORCID 0000-0001-7686-4866

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Previous research demonstrated the distribution of distinct microbial communities in the equine hindgut surrounding the pelvic flexure. The current study evaluated gene expression in epithelial tissues surrounding the pelvic flexure to characterize patterns that might correlate with microbial distribution. Gene expression was determined by analyzing RNA sequence data from the pelvic flexure, the left and right ventral colon, and the left and right dorsal colon. An average of 18,330 genes were expressed across the five tissues sampled. Most of the genes showed some level of expression in all five tissues. Tissue-restricted patterns of expression were also observed. Genes with restricted expression in the left ventral and left dorsal colons have communication, signaling, and regulatory functions that correlate with their known physiology. In contrast, genes expressed exclusively in the pelvic flexure have diverse functions. The ontology of genes differentially expressed between the pelvic flexure and the surrounding tissues was associated with immune functions and signaling processes. Despite being non-significant, these enrichment trends were reinforced by the functions of statistically significant expression differences between tissues of the hindgut. These results provide insight into the physiology of the equine hindgut epithelium that might influence the microbiota and its distribution.

Indexed as

equinegastrointestinalhindguthomeostasismicrobiomepelvic flexureRNA sequencing

Identifiers

PMID39199837
PMCPMC11350661

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.