ArticleNature communications2024
Label-free, real-time monitoring of membrane binding events at zeptomolar concentrations using frequency-locked optical microresonators.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Sub-parts-per-billion CONature communications · 2026Article
- Bound States in the Continuum Metasurfaces for Biomolecular Detection: A Review of Advances and Applications.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- Engineering graphene oxide interfaces for electrochemical biosensing of biomolecules, cells, and organoids.Nano convergence · 2026Review
- Free-Space-Coupled Frequency-Locked Microtoroid Resonators with Reactive Polymer Functionalization for Part-Per-Trillion Gas Detection.Laser & photonics reviews · 2026Article
- Liquid photonic-molecule microlasers for ultrasensitive biosensing.Nature communications · 2026Article
- Accelerating GPCR drug discovery through computation and experiment integrated with direct detection of ligand binding events.npj drug discovery · 2026Review
- FLOWER: a frequency-locked optical whispering evanescent resonator for label-free molecular detection.Chemical communications (Cambridge, England) · 2025Review
- Frequency locked whispering evanescent resonator (FLOWER) for biochemical sensing applications.Nature protocols · 2025Review
- Optical biosensors for diagnosing neurodegenerative diseases.Npj biosensing · 2025Review
- Agonist activation to open the Gα subunit of the GPCR-G protein precoupled complex defines functional agonist activation of TAS2R5.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Steviol rebaudiosides bind to four different sites of the human sweet taste receptor (T1R2/T1R3) complex explaining confusing experiments.Communications chemistry · 2024Article
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7 authors.
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Abstract
G-protein coupled receptors help regulate cellular function and communication, and are targets of small molecule drug discovery efforts. Conventional techniques to probe these interactions require labels and large amounts of receptor to achieve satisfactory sensitivity. Here, we use frequency-locked optical microtoroids for label-free characterization of membrane interactions in vitro at zeptomolar concentrations for the kappa opioid receptor and its native agonist dynorphin A 1-13, as well as big dynorphin (dynorphin A and dynorphin B) using a supported biomimetic membrane. The measured affinity of the agonist dynorphin A 1-13 to the κ-opioid receptor was also measured and found to be 3.1 nM. Radioligand assays revealed a dissociation constant in agreement with this value (1.1 nM). The limit of detection for the κOR/DynA 1-13 was calculated as 180 zM. The binding of Cholera Toxin B-monosialotetrahexosyl ganglioside was also monitored in real-time and an equilibrium dissociation constant of 1.53 nM was found. Our biosensing platform provides a method for highly sensitive real-time characterization of membrane embedded protein binding kinetics that is rapid and label-free, for drug discovery and toxin screening among other applications.
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