Evidence map›Paper›PMID 39198318›Full record

ArticlePharmaceutical research2024

MR Molecular Image Guided Treatment of Pancreatic Cancer with Targeted ECO/miR-200c Nanoparticles in Immunocompetent Mouse Tumor Models.

Victoria Laney, Ryan Hall, Xueer Yuan, Emma Hampson, Augusta Halle, Grace Yeung, Kristen-Weber Bonk, Suneel Apte, Jordan Winter, Ruth Keri and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pharmaceutical research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. EMT and cancer: what clinicians should know.Nature reviews. Clinical oncology · 2025
    Review
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Victoria LaneyDepartment of Biomedical Engineering, Case Western Reserve University, Wickenden 427, 10900 Euclid Avenue, Cleveland, OH, 44106, USA.
Ryan HallDepartment of Biomedical Engineering, Case Western Reserve University, Wickenden 427, 10900 Euclid Avenue, Cleveland, OH, 44106, USA.
Xueer YuanDepartment of Biomedical Engineering, Case Western Reserve University, Wickenden 427, 10900 Euclid Avenue, Cleveland, OH, 44106, USA.
Emma HampsonDepartment of Biomedical Engineering, Case Western Reserve University, Wickenden 427, 10900 Euclid Avenue, Cleveland, OH, 44106, USA.
Augusta HalleDepartment of Biomedical Engineering, Case Western Reserve University, Wickenden 427, 10900 Euclid Avenue, Cleveland, OH, 44106, USA.
Grace YeungDepartment of Biomedical Engineering, Case Western Reserve University, Wickenden 427, 10900 Euclid Avenue, Cleveland, OH, 44106, USA.
Kristen-Weber BonkLerner Research Institute, Cleveland Clinic, Cleveland, OH, 44106, USA.
Suneel ApteLerner Research Institute, Cleveland Clinic, Cleveland, OH, 44106, USA.
Jordan WinterSurgical Oncology, The University Hospitals of Cleveland, Cleveland, OH, 44106, USA.
Ruth KeriLerner Research Institute, Cleveland Clinic, Cleveland, OH, 44106, USA.
Zheng-Rong LuDepartment of Biomedical Engineering, Case Western Reserve University, Wickenden 427, 10900 Euclid Avenue, Cleveland, OH, 44106, USA. zxl125@case.edu.

Funding

Smart nanoparticles regulating oncogenic IncRNA for breast cancer therapyR01CA235152 · NCI · CASE WESTERN RESERVE UNIVERSITY · PI ZHENG-RONG LU, William Schiemann · 2019 to 2026
$3.2M
NCI NIH HHS R01 CA235152NIH HHS R01 CA235152
6 · The paper itself

Abstract

objectivePancreatic ductal adenocarcinoma (PDAC) is characterized by desmoplasia due to increased deposition of extracellular matrix (ECM) proteins. This work investigates the efficacy of targeted ECO/miR-200c nanoparticles (ELNP) on ECM remodeling in PDAC and tumor proliferation with MR molecular imaging (MRMI) with MT218 in immunocompetent mouse models.

methodsThe miR-200c mediated regulation of EMT markers was measured in PDAC cells in vitro. Wild-type mice bearing mutated KRAS-driven KPC subcutaneous or orthotopic tumors were dosed weekly with RGD-ELNP/miR-200c at 1 mg-RNA/kg for a total of 4 doses. We utilized MT218-MRMI to non-invasively monitor the alteration of tumor ECM EDN-FN levels by miR-200c and tumor response to the treatment. The changes were also validated by posthumous histopathology.

resultsTransfection of PDAC cells with ELNP/miR-200c downregulated the expression of FN1 and EDB-FN and some mesenchymal markers, inhibiting 3D spheroid formation and migration of KPC PDAC cells. RGD-ELNP/miR-200c treatment resulted in significant signal reduction in the MT218 enhanced MRMI images of both subcutaneous and orthotopic KPC tumors compared to those prior to treatment and treated with a non-specific control. MT218-MRMI results were suggestive of EDB-FN downregulation in tumors, which was later confirmed by immunohistochemistry. Tumor growth in subcutaneous tumors was significantly attenuated with RGD-ELNP/miR-200c and was an observed trend in orthotopic tumors. Substantial necrosis and remodeling were observed in both models treated with RGD-ELNP/miR-200c based on H&E staining.

conclusionThese results demonstrate the feasibility of RGD-ELNP/miR-200c in modulating PDAC ECM and restraining tumor growth and the utility of MT218-MRMI for non-invasively monitoring miR-200c efficacy.

Indexed as

Carcinoma, Pancreatic DuctalMagnetic Resonance ImagingMicroRNAsNanoparticlesPancreatic NeoplasmsAnimalsCell Line, TumorCell ProliferationDisease Models, AnimalHumansMiceMolecular ImagingMicroRNAsMirn200 microRNA, mouseECOmagnetic resonance molecular imagingmiR-200cMT218pancreatic cancer

Identifiers

PMID39198318
PMCPMC11436418

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.