Evidence map›Paper›PMID 39197448›Full record

ArticleCell2024

Opposing GPCR signaling programs protein intake setpoint in Drosophila.

Guangyan Wu, Tianji Ma, Clare E Hancock, Santiago Gonzalez, Binod Aryal, Sharon Vaz, Gabrielle Chan, Madison Palarca-Wong, Nick Allen, Chan-I Chung and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Frontiers in endocrinology · 2026
    Article
  6. Article
  7. Sexual dimorphism in the complete connectome of thebioRxiv : the preprint server for biology · 2025
    Article
  8. Stress-induced preference for antioxidants byProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  9. Article
  10. Review
  11. Article
  12. Review
  13. Article
  14. Article
  15. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Guangyan WuDepartment of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA.
Tianji MaDepartment of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA.
Clare E HancockDepartment of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA.
Santiago GonzalezDepartment of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA.
Binod AryalDepartment of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA.
Sharon VazDepartment of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA.
Gabrielle ChanDepartment of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA.
Madison Palarca-WongDepartment of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA.
Nick AllenDepartment of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA.
Chan-I ChungDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158, USA.
Xiaokun ShuDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158, USA.
Qili LiuDepartment of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA; Kavli Institute for Fundamental Neuroscience, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: qili.liu@ucsf.edu.

Funding

EQUIPMENT for Designing a new class of fluorescent reporters for imaging dynamic cell signaling in live animalsR35GM131766 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI SHU, XIAOKUN · 2019 to 2025
$4.0M
Mechanism and Modulation of the homeostatic setpoint for protein feedingR01NS123890 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LIU, QILI · 2021 to 2025
$2.3M
NIGMS NIH HHS R35 GM131766NINDS NIH HHS R01 NS123890
6 · The paper itself

Abstract

Animals defend a target level for their fundamental needs, including food, water, and sleep. Deviation from the target range, or "setpoint," triggers motivated behaviors to eliminate that difference. Whether and how the setpoint itself is encoded remains enigmatic for all motivated behaviors. Employing a high-throughput feeding assay in Drosophila, we demonstrate that the protein intake setpoint is set to different values in male, virgin female, and mated female flies to meet their varying protein demands. Leveraging this setpoint variability, we found, remarkably, that the information on the intake setpoint is stored within the protein hunger neurons as the resting membrane potential. Two RFamide G protein-coupled receptor (GPCR) pathways, by tuning the resting membrane potential in opposite directions, coordinately program and adjust the protein intake setpoint. Together, our studies map the protein intake setpoint to a single trackable physiological parameter and elucidate the cellular and molecular mechanisms underlying setpoint determination and modulation.

Indexed as

Drosophila melanogasterDrosophila ProteinsNeuronsReceptors, G-Protein-CoupledSignal TransductionAnimalsFeeding BehaviorFemaleMaleNeuropeptidesDrosophila ProteinsNeuropeptidesReceptors, G-Protein-CoupledGPCR signalinghomeostasismotivated behaviorprotein intake setpointresting membrane potentialRFamide

Identifiers

PMID39197448
PMCPMC11437785

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.