ArticleClinical mass spectrometry (Del Mar, Calif.)
Multiplexed analysis of steroid hormones in saliva by LC-MS/MS with 2-hydrazinopyridine derivatization.
Article in Clinical mass spectrometry (Del Mar, Calif.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Combination of magnetic bead extraction and LC-MS/MS detection for the detection of saliva cortisol.Analytical and bioanalytical chemistry · 2026Article
- An investigation of 2-hydrazinyl-1,3-benzothiazole, 4-amino-4H-1,2,4-triazole and 2-hydrazinyl-1H-benzimidazole applicability as derivatization reagents for HPLC-HRMS determination of steroid hormones in urine.Frontiers in chemistry · 2026Article
- Synthesis, Reactions, and Agrochemical Studies of New 4,6-Diaryl-2-hydrazinylnicotinonitriles.International journal of molecular sciences · 2025Article
- Non-invasive assessment of free steroid hormones: development of a high-throughput LC-MS/MS method for salivary steroid hormone quantification.Analytical and bioanalytical chemistry · 2025Article
- Challenges and recent advances in quantitative mass spectrometry-based metabolomics.Analytical science advances · 2024Review
- Using LC-MS/MS to Determine Salivary Steroid Reference Intervals in a European Older Adult Population.Metabolites · 2023Article
- Monitoring Farmed Fish Welfare by Measurement of Cortisol as a Stress Marker in Fish Feces by Liquid Chromatography Coupled with Tandem Mass Spectrometry.Molecules (Basel, Switzerland) · 2022Article
- Progress and Challenges in Quantifying Carbonyl-Metabolomic Phenomes with LC-MS/MS.Molecules (Basel, Switzerland) · 2021Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: LC-MS/MS methods for multiplexed analysis of steroid hormones in saliva are useful research tools in endocrinology, but require sensitive detection. Objective: To explore the use of hydrazide and hydrazine derivatization to improve sensitivity of detection for ketosteroids. On development and validation of a sample preparation method based on robot pipetting in the 96-well format we intended to then establish normal reference ranges for women and men, with saliva collected both in the early morning and late at night. Method: Four hydrazides and one hydrazine were evaluated for their effectiveness as derivatization reagents based on the comparative signal response and efficacy of LC separation of five ketosteroid hydrazones via a methanol gradient mixed with either 0.2% formic acid or 0.1% ammonium hydroxide. Processing of saliva via liquid-liquid extraction (LLE) was optimized by examining variations in both extraction solvent polarity and protein precipitation reagents intended to inhibit emulsion. Results: LLE using 10 % butanol in methyl Conclusion: Tannic acid is a novel and effective protein precipitation reagent in bioanalytical sample preparation. The validated method was used in the establishment of reference ranges, the success of which indicated that the method is suitable for Cushing's screening and has potential as a novel analytical research tool.
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